Transcriptomics Analysis of the Chinese Pear Pathotype of Alternaria alternata Gives Insights into Novel Mechanisms of HSAF Antifungal Activities.
Transcriptomics Analysis of the Chinese Pear Pathotype of Alternaria alternata Gives Insights into Novel Mechanisms of HSAF Antifungal Activities.
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DOI：
10.3390/ijms19071841
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发表时间：
2018-06-22
影响因子：
5.6
通讯作者：
Cao H
中科院分区：
文献类型：
--
作者：
He F;Li B;Ai G;Kange AM;Zhao Y;Zhang X;Jia Y;Dou D;Liu F;Cao H
梨黑斑病是我国南方梨上的一种致死性病原菌。热稳定活性因子（HSAF）是由产酶溶杆菌等微生物产生的一种多环四酸酯大环内酰胺（PTM），对多种丝状真菌具有广谱抗真菌活性。本研究评价了HSAF对A. alternata的抑菌机理进行了初步探讨。交替HSAF对A. alternata以剂量依赖性方式。转录组学分析显示，HSAF处理导致了广泛的基因表达的变化，其中3729个基因被上调，3640个基因被下调。此外，我们观察到HSAF处理破坏了A中的多种信号网络和必需的细胞代谢。Alternata的主要功能包括AMPK信号通路、鞘脂代谢和信号通路、碳代谢和TCA（三羧酸）循环、细胞周期、氮代谢、细胞壁合成和关键枢纽蛋白磷酸酶2A（PP2A）。这些观察结果表明，HSAF破坏代谢网络，并最终诱导A.交替深入了解HSAF对丝状真菌的抑菌机理，将有助于进一步确定HSAF的直接作用靶标，并将其开发为新型生物杀菌剂。
Alternaria alternata (Fries) Keissler is a lethal pear pathogen that causes leaf black spot disease of pear in Southern China. Heat-stable activity factor (HSAF) is a polycyclic tetramate macrolactam (PTM) produced by Lysobacter enzymogenes and many other microbes with a broad-spectrum antifungal activity against many filamentous fungi. In this study, we evaluated the antifungal effect of HSAF against A. alternata and proposed its antifungal mechanism in A. alternata. We report that HSAF inhibited the mycelial growth of A. alternata in a dose-dependent manner. Transcriptomics analysis revealed that HSAF treatment resulted in an expression alteration of a wide range of genes, with 3729 genes being up-regulated, and 3640 genes being down-regulated. Furthermore, we observed that HSAF treatment disrupted multiple signaling networks and essential cellular metabolisms in A. alternata, including the AMPK signaling pathway, sphingolipid metabolism and signaling pathway, carbon metabolism and the TCA (tricarboxylic acid) cycle, cell cycle, nitrogen metabolism, cell wall synthesis and a key hub protein phosphatase 2A (PP2A). These observations suggest that HSAF breaches metabolism networks and ultimately induces increased thickness of the cell wall and apoptosis in A. alternata. The improved understanding of the antifungal mechanism of HSAF against filamentous fungi will aid in the future identification of the direct interaction target of HSAF and development of HSAF as a novel bio-fungicide.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
14.9
作者：
Finn RD;Clements J;Arndt W;Miller BL;Wheeler TJ;Schreiber F;Bateman A;Eddy SR
通讯作者：
Eddy SR
影响因子：
5
作者：
Han, Yong;Wang, Yan;Tombosa, Simon;Wright, Stephen;Huffman, Justin;Yuen, Gary;Qian, Guoliang;Liu, Fengquan;Shen, Yuemao;Du, Liangcheng
通讯作者：
Du, Liangcheng
影响因子：
3.8
作者：
He, Feng;Zhang, Xiong;Dou, Daolong
通讯作者：
Dou, Daolong