Absence of the aflatoxin biosynthesis gene, norA, allows accumulation of deoxyaflatoxin B1 in Aspergillus flavus cultures.
Absence of the aflatoxin biosynthesis gene, norA, allows accumulation of deoxyaflatoxin B1 in Aspergillus flavus cultures.
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DOI：
10.1111/j.1574-6968.2010.01914.x
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发表时间：
2010-04
影响因子：
2.1
通讯作者：
Townsend CA
中科院分区：
文献类型：
--
作者：
Ehrlich KC;Chang PK;Scharfenstein LL Jr;Cary JW;Crawford JM;Townsend CA
从常见的中间体O-甲基杂色曲霉毒素(OMST)中生物合成剧毒和致癌的黄曲霉毒素被认为只需要细胞色素P450单加氧酶ORDA(AFLQ)。我们现在提供的证据表明，黄曲霉中黄曲霉毒素生物合成基因簇编码的芳醇脱氢酶NorA(AflE)影响黄曲霉毒素生物合成最后步骤中黄曲霉毒素的积累。具有非活性NORA的突变体产生的黄曲霉毒素B1(AFB1)数量减少，但一种新的代谢物脱氧AFB1的数量增加。为了解释这一结果，我们认为，在没有NorA的情况下，AFB1还原产物黄曲霉毒素会产生，并在酸性介质中很容易脱水成脱氧AFB1，使我们能够观察到这种在野生型黄曲霉中产生的微小毒素。
Biosynthesis of the highly toxic and carcinogenic aflatoxins in select Aspergillus species from the common intermediate O-methylsterigmatocystin (OMST) has been postulated to require only the cytochrome P450 monooxygenase, OrdA (AflQ). We now provide evidence that the aryl alcohol dehydrogenase NorA (AflE) encoded by the aflatoxin biosynthetic gene cluster in A. flavus affects the accumulation of aflatoxins in the final steps of aflatoxin biosynthesis. Mutants with inactive norA produced reduced quantities of aflatoxin B1 (AFB1), but elevated quantities of a new metabolite, deoxyAFB1. To explain this result, we suggest that, in the absence of NorA, the AFB1 reduction product, aflatoxicol, is produced and is readily dehydrated to deoxyAFB1 in the acidic medium, enabling us to observe this otherwise minor toxin produced in wild-type A. flavus.
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影响因子：
5.6
作者：
Ehrlich, Kenneth C.;Scharfenstein, Leslie L., Jr.;Montalbano, Beverly G.;Chang, Perng-Kuang
通讯作者：
Chang, Perng-Kuang
影响因子：
15
作者：
Udwary, DW;Casillas, LK;Townsend, CA
通讯作者：
Townsend, CA
影响因子：
4.4
作者：
BHATNAGAR, D;MCCORMICK, SP;HILL, RA
通讯作者：
HILL, RA
影响因子：
4.4
作者：
NAKAZATO, M;MOROZUMI, S;KASAI, N
通讯作者：
KASAI, N
影响因子：
4.4
作者：
Prieto, R;Yousibova, GL;Woloshuk, CP
通讯作者：
Woloshuk, CP