Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus.
Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus.
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DOI：
10.1038/s42003-021-02134-w
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发表时间：
2021-05-24
影响因子：
5.9
通讯作者：
Lomonossoff GP
中科院分区：
文献类型：
--
作者：
Castells-Graells R;Ribeiro JRS;Domitrovic T;Hesketh EL;Scarff CA;Johnson JE;Ranson NA;Lawson DM;Lomonossoff GP
许多病毒衣壳在其宿主细胞中经历精心设计的成熟过程以产生感染性病毒体，这些仍然知之甚少。作为研究病毒成熟的工具，我们在本氏烟草中瞬时表达了昆虫病毒角裸壳虫omega病毒（NωV）的衣壳蛋白，并且能够通过改变表达时间从浸润的叶片中纯化未成熟的前衣壳和成熟的衣壳。对植物产生的原衣壳和成熟衣壳的Cryo-EM分析，分别达到6.6 μ m和2.7 μ m的分辨率，揭示了除了大规模的刚体运动外，亚基的内部区域在成熟过程中被广泛重塑，产生了自催化裂解和感染性所需的活性位点。成熟颗粒在其裂解膜的能力方面具有生物活性，并且具有与真实病毒基本相同的结构。忠实地概括和可视化植物中复杂的成熟过程的能力，包括衣壳蛋白的自催化裂解，揭示了成熟过程中亚基的~30个构象旋转以及每个亚基的N和C末端螺旋区域的构象重排。Castells-Graells等人证明了昆虫病毒角裸壳虫omega病毒（NωV）衣壳在植物模型中的成熟。这表明植物是跨界研究真核病毒的有用工具，并且它们鉴定了NωV衣壳成熟的新特征。
Many virus capsids undergo exquisitely choreographed maturation processes in their host cells to produce infectious virions, and these remain poorly understood. As a tool for studying virus maturation, we transiently expressed the capsid protein of the insect virus Nudaurelia capensis omega virus (NωV) in Nicotiana benthamiana and were able to purify both immature procapsids and mature capsids from infiltrated leaves by varying the expression time. Cryo-EM analysis of the plant-produced procapsids and mature capsids to 6.6 Å and 2.7 Å resolution, respectively, reveals that in addition to large scale rigid body motions, internal regions of the subunits are extensively remodelled during maturation, creating the active site required for autocatalytic cleavage and infectivity. The mature particles are biologically active in terms of their ability to lyse membranes and have a structure that is essentially identical to authentic virus. The ability to faithfully recapitulate and visualize a complex maturation process in plants, including the autocatalytic cleavage of the capsid protein, has revealed a ~30 Å translation-rotation of the subunits during maturation as well as conformational rearrangements in the N and C-terminal helical regions of each subunit. Castells-Graells et al. demonstrate the maturation of insect virus Nudaurelia capensis omega virus (NωV) capsids in a plant model. This shows plants are a useful tool in the study of eukaryotic viruses across Kingdoms, and they identify new features in the maturation of the NωV capsid.
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影响因子：
48
作者：
Barad BA;Echols N;Wang RY;Cheng Y;DiMaio F;Adams PD;Fraser JS
通讯作者：
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
Acta crystallographica. Section D, Structural biology
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/j.ejpb.2020.08.012
发表时间：
2020-10-01
影响因子：
4.9
作者：
Berardi, Alberto;Castells-Graells, Roger;Lomonossoff, George P.
通讯作者：
Lomonossoff, George P.