Nucleus Accumbens Chemogenetic Inhibition Suppresses Amphetamine-Induced Ultrasonic Vocalizations in Male and Female Rats.
Nucleus Accumbens Chemogenetic Inhibition Suppresses Amphetamine-Induced Ultrasonic Vocalizations in Male and Female Rats.
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DOI：
10.3390/brainsci11101255
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发表时间：
2021-09-22
期刊：
影响因子：
3.3
通讯作者：
Mahler SV
中科院分区：
文献类型：
--
作者：
Lawson KA;Flores AY;Hokenson RE;Ruiz CM;Mahler SV
成年大鼠发出与其情感状态相关的超声发声（USVs），可能在行为神经科学实验期间提供有关其主观体验的信息。如果是这样的话，USV可能会在侵入性动物临床前研究和人类研究之间提供一个重要的联系，其中主观状态可以很容易地查询。在这里，我们使用急性安非他明（2 mg/kg）在雄性和雌性Long Evans大鼠中诱导USV，并询问如何可逆地抑制使用设计药物（DREADDs）专门激活的设计受体的丘脑核神经元影响USV的产生。我们使用“Deepsqueak”软件分析了USV特征，并将检测到的呼叫手动分类为四个先前定义的亚型。我们发现，在相同大鼠中，相对于溶媒，DREADD激动剂氯氮平-n-氧化物的全身给药抑制了频率调制和含颤音的USV的数量，而不影响高频、未调制（平坦）USV，也不影响观察到的少量低频USV。使用化学遗传学，这些结果因此证实，丘脑核神经元是必不可少的生产苯丙胺诱导的频率调制USVs。他们还支持进一步研究这些呼叫的特征和子类别的前提，作为神经操作的主观影响的窗口，具有潜在的未来临床应用。
Adult rats emit ultrasonic vocalizations (USVs) related to their affective states, potentially providing information about their subjective experiences during behavioral neuroscience experiments. If so, USVs might provide an important link between invasive animal preclinical studies and human studies in which subjective states can be readily queried. Here, we induced USVs in male and female Long Evans rats using acute amphetamine (2 mg/kg), and asked how reversibly inhibiting nucleus accumbens neurons using designer receptors exclusively activated by designer drugs (DREADDs) impacts USV production. We analyzed USV characteristics using “Deepsqueak” software, and manually categorized detected calls into four previously defined subtypes. We found that systemic administration of the DREADD agonist clozapine-n-oxide, relative to vehicle in the same rats, suppressed the number of frequency-modulated and trill-containing USVs without impacting high frequency, unmodulated (flat) USVs, nor the small number of low-frequency USVs observed. Using chemogenetics, these results thus confirm that nucleus accumbens neurons are essential for production of amphetamine-induced frequency-modulated USVs. They also support the premise of further investigating the characteristics and subcategories of these calls as a window into the subjective effects of neural manipulations, with potential future clinical applications.
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DOI：
10.1073/pnas.0700293104
发表时间：
2007-03-20
影响因子：
11.1
作者：
Armbruster, Blaine N.;Li, Xiang;Roth, Bryan L.
通讯作者：
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影响因子：
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作者：
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3
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作者：
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影响因子：
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通讯作者：
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