The fungal myosin I is essential for Fusarium toxisome formation.
The fungal myosin I is essential for Fusarium toxisome formation.
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真菌肌球蛋白 I 对于镰刀菌毒素体的形成至关重要
DOI：
10.1371/journal.ppat.1006827
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发表时间：
2018-01
期刊：
影响因子：
6.7
通讯作者：
Ma Z
中科院分区：
文献类型：
--
作者：
Tang G;Chen Y;Xu JR;Kistler HC;Ma Z
肌球蛋白-I分子马达被提出在几个细胞过程中作为膜和肌动蛋白细胞骨架之间的连接物，但它们在真菌次级代谢产物的生物合成中的作用仍然难以捉摸。在这里，我们发现，禾谷镰刀菌（FgMyo 1）的肌球蛋白I，镰刀菌头枯病的病原体，真菌毒素的生物合成中起着关键作用。小分子非那普利对肌球蛋白I的抑制导致脱氧雪腐镰刀菌烯醇（DON）生物合成的显著减少。FgMyo 1还通过与核糖体相关蛋白FgAsc 1相互作用来控制DON生物合成酶Tri 1的翻译。通过突变E420 K、下调FgMyo 1表达或缺失FgAsc 1破坏FgMyo 1的ATP酶活性导致Tri 1翻译减少。DON生物合成酶Tri 1和Tri 4主要定位于被称为毒素体的亚细胞结构，以响应真菌毒素诱导，并且FgMyo 1相互作用蛋白肌动蛋白参与毒素体形成。肌动蛋白聚合破坏剂latrunculin A抑制毒素体组装。与这一观察结果一致，肌动蛋白相关蛋白FgPrk 1和FgEnd 3的缺失也导致减少的毒素体形成。出乎意料的是，FgMyo 1-肌动蛋白细胞骨架不参与测试的另一种次级代谢物的生物合成。总之，这项研究揭示了一个新的功能，肌球蛋白I在调节丝状真菌真菌毒素的生物合成。
Myosin-I molecular motors are proposed to function as linkers between membranes and the actin cytoskeleton in several cellular processes, but their role in the biosynthesis of fungal secondary metabolites remain elusive. Here, we found that the myosin I of Fusarium graminearum (FgMyo1), the causal agent of Fusarium head blight, plays critical roles in mycotoxin biosynthesis. Inhibition of myosin I by the small molecule phenamacril leads to marked reduction in deoxynivalenol (DON) biosynthesis. FgMyo1 also governs translation of the DON biosynthetic enzyme Tri1 by interacting with the ribosome-associated protein FgAsc1. Disruption of the ATPase activity of FgMyo1 either by the mutation E420K, down-regulation of FgMyo1 expression or deletion of FgAsc1 results in reduced Tri1 translation. The DON biosynthetic enzymes Tri1 and Tri4 are mainly localized to subcellular structures known as toxisomes in response to mycotoxin induction and the FgMyo1-interacting protein, actin, participates in toxisome formation. The actin polymerization disruptor latrunculin A inhibits toxisome assembly. Consistent with this observation, deletion of the actin-associated proteins FgPrk1 and FgEnd3 also results in reduced toxisome formation. Unexpectedly, the FgMyo1-actin cytoskeleton is not involved in biosynthesis of another secondary metabolite tested. Taken together, this study uncovers a novel function of myosin I in regulating mycotoxin biosynthesis in filamentous fungi.
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DOI：
10.1083/jcb.144.6.1203
发表时间：
1999-03-22
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cope MJ;Yang S;Shang C;Drubin DG
通讯作者：
Drubin DG
影响因子：
4.6
作者：
Chen Y;Santos A;Wang Y;Kumeria T;Ho D;Li J;Wang C;Losic D
通讯作者：
Losic D
影响因子：
8.4
作者：
Fernandez-Aguado, M.;Martin, J. F.;Ullan, R. V.
通讯作者：
Ullan, R. V.
影响因子：
4.2
作者：
Audenaert K;Vanheule A;Höfte M;Haesaert G
通讯作者：
Haesaert G
影响因子：
2.8
作者：
CAPPELLINI, RA;PETERSON, JL
通讯作者：
PETERSON, JL