A microfluidic device for partial cell separation and deformability assessment
A microfluidic device for partial cell separation and deformability assessment
复制标题
用于部分细胞分离和变形性评估的微流体装置
作者：
D. Pinho;T. Yaginuma;R. Lima
微循环中的血液流动显示了几种有趣的现象，这些现象可用于开发用于连续流动中的血液分离和分析的微流体装置。在这项研究中，我们提出了一种新的连续微流控装置的部分提取红细胞（RBC）和随后的测量红细胞变形性。为此，我们使用聚二甲基硅氧烷（PDMS）的微通道具有不同的收缩（25%，50%和75%），以研究其对无细胞层（CFL）的厚度和分离效率的影响。通过使用图像分析技术的组合，我们能够自动测量CFL宽度之前和之后的人工收缩。结果表明，CFL的宽度增加与收缩的增强，并有助于部分细胞分离。红细胞变形指数的后续测量显示，变形程度取决于收缩几何形状和细胞分离模块后的血细胞比容。所提出的微流体装置可以容易地转变成简单、廉价且方便的临床工具，其能够在单个装置中执行RBC分离和变形性分析。这将消除对外部样品处理的需要，从而减少相关的劳动力成本和潜在的人为错误。
Blood flow in microcirculation shows several interesting phenomena that can be used to develop microfluidic devices for blood separation and analysis in continuous flow. In this study we present a novel continuous microfluidic device for partial extraction of red blood cells (RBCs) and subsequent measurement of RBC deformability. For this purpose, we use polydimethylsiloxane (PDMS) microchannels having different constrictions (25%, 50% and 75%) to investigate their effect on the cell-free layer (CFL) thickness and separation efficiency. By using a combination of image analysis techniques we are able to automatically measure the CFL width before and after an artificial constriction. The results suggest that the CFL width increases with enhancement of the constriction and contributes to partial cell separation. The subsequent measurements of RBCs deformation index reveal that the degree of deformation depends on the constriction geometries and hematocrit after the cell separation module. The proposed microfluidic device can be easily transformed into a simple, inexpensive and convenient clinical tool able to perform both RBC separation and deformability analysis in one single device. This would eliminate the need for external sample handling and thus reducing associated labor costs and potential human errors.
影响因子：
6.1
作者：
Hou, Han Wei;Bhagat, Ali Asgar S.;Lim, Chwee Teck
通讯作者：
Lim, Chwee Teck
影响因子：
7.4
作者：
Shevkoplyas, SS;Yoshida, T;Bitensky, MW
通讯作者：
Bitensky, MW