Posttranslational modifications of alpha tubulin: detyrosination and acetylation differentiate populations of interphase microtubules in cultured cells.
Posttranslational modifications of alpha tubulin: detyrosination and acetylation differentiate populations of interphase microtubules in cultured cells.
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DOI：
10.1083/jcb.106.4.1213
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发表时间：
1988-04
期刊：
影响因子：
--
通讯作者：
Piperno G
中科院分区：
文献类型：
--
作者：
Bulinski JC;Richards JE;Piperno G
先前已描述了富含翻译后去酪氨酸（Gundersen, G. G., M. H. Kalnoski, and J. C. Bulinski. 1984. Cell. 38:779）或乙酰化（Piperno, G., M. Le Dizet, and X. Chang. 1987. J. Cell Biol. 104:298）的微管子集，α微管蛋白在间期培养的细胞中。在这项研究中，对这些少量微管群的免疫荧光比较表明，在非洲绿猴肾上皮细胞（TC-7系）中，富含去酪氨酸微管蛋白的微管群实际上与富含乙酰化α微管蛋白的微管群一致。然而，在某些细胞类型中，例如人 HeLa 或有袋动物 PtK-2 细胞，在微管中仅可检测到一种翻译后修饰形式的微管蛋白，即乙酰化或去酪氨酸化形式。在 TC-7 细胞中，尽管存在两种修饰，但在从微管解聚处理或有丝分裂中恢复细胞后，观察到富含去酪氨酸和乙酰化微管蛋白的微管再现的不同模式和动力学。因此，培养细胞中存在少量微管，这些细胞含有以一种或两种方式修饰的微管蛋白水平升高。虽然这两种修饰主要发生在同一微管子集上，但它们在体内的形成模式不同。
Subsets of microtubules enriched in posttranslationally detyrosinated (Gundersen, G. G., M. H. Kalnoski, and J. C. Bulinski. 1984. Cell. 38:779) or acetylated (Piperno, G., M. Le Dizet, and X. Chang. 1987. J. Cell Biol. 104:298), alpha tubulin have previously been described in interphase cultured cells. In this study an immunofluorescence comparison of these minor populations of microtubules revealed that, in African green monkey kidney epithelial cells (TC-7 line), the population of microtubules enriched in detyrosinated tubulin was virtually coincident with the population enriched in acetylated alpha tubulin. In some cell types, however, such as human HeLa or marsupial PtK-2 cells, only one posttranslationally modified form of tubulin, i.e., acetylated or detyrosinated, respectively, was detectable in microtubules. In TC-7 cells, although both modifications were present, dissimilar patterns and kinetics of reappearance of microtubules enriched in detyrosinated and acetylated tubulin were observed after recovery of cells from microtubule-depolymerizing treatments or from mitosis. Thus, a minor population of microtubules exists in cultured cells that contains an elevated level of tubulin modified in either one or two ways. While these two modifications occur primarily on the same subset of microtubules, they differ in their patterns of formation in vivo.
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影响因子：
7.8
作者：
SCHULZE, E;KIRSCHNER, M
通讯作者：
KIRSCHNER, M
影响因子：
7.8
作者：
GEUENS, G;GUNDERSEN, GG;DEBRABANDER, M
通讯作者：
DEBRABANDER, M
影响因子：
2.9
作者：
SANDOVAL, IV;CUATRECASAS, P
通讯作者：
CUATRECASAS, P
DOI：
10.1083/jcb.101.6.2085
发表时间：
1985-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Piperno G;Fuller MT
通讯作者：
Fuller MT
DOI：
10.1083/jcb.101.5.1763
发表时间：
1985-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Caron JM;Jones AL;Kirschner MW
通讯作者：
Kirschner MW