Guanosine cyclic 3',5'-phosphate dependent protein kinase, a chimeric protein homologous with two separate protein families.
Guanosine cyclic 3',5'-phosphate dependent protein kinase, a chimeric protein homologous with two separate protein families.
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鸟苷环 3,5-磷酸依赖性蛋白激酶，一种与两个独立蛋白家族同源的嵌合蛋白。
作者：
K. Takio;R. D. Wade;S. B. Smith;E. Krebs;K. Walsh;K. Titani
牛肺cgmp依赖性蛋白激酶的氨基酸序列已被确定通过降解和排列两个主要重叠肽组在蛋氨酸基或精氨酸基残基裂解产生。该蛋白在单个N -乙酰化链中含有670个残基，分子量为76 331。分子的功能被认为是在六个片段的序列，可能对应于四个折叠域。从氨基端来看，第一部分与蛋白质的二聚化特性有关。第二段和第三段似乎是从祖先的串联内部基因复制进化而来的，产生了双cgmp结合结构域，这些结构域与camp依赖性蛋白激酶调控亚基的双结构域和大肠杆菌分解代谢基因激活物的camp结合结构域同源。第四和第五段可能包含一个结构域，该结构域与camp依赖性蛋白激酶、钙依赖性磷酸化酶b激酶和某些致癌病毒蛋白酪氨酸激酶的催化亚基同源。cgmp依赖性蛋白激酶的调节性氨基末端一半似乎与一个以多种目的结合cAMP的较小蛋白家族有关，而催化性羧基末端一半则与一个具有不同特异性和对调节剂不同敏感性的蛋白激酶家族有关。这些数据表明，祖先基因剪接事件可能参与了两个蛋白质家族的融合，从而产生了这种嵌合酶的变构特性。
The amino acid sequence of bovine lung cGMP-dependent protein kinase has been determined by degradation and alignment of two primary overlapping sets of peptides generated by cleavage at methionyl or arginyl residues. The protein contains 670 residues in a single N alpha-acetylated chain corresponding to a molecular weight of 76 331. The function of the molecule is considered in six segments of sequence which may correspond to four folding domains. From the amino terminus, the first segment is related to the dimerizing property of the protein. The second and third segments appear to have evolved from an ancestral tandem internal gene duplication, generating twin cGMP-binding domains which are homologous to twin domains in the regulatory subunits of cAMP-dependent protein kinase and to the cAMP-binding domain of the catabolite gene activator of Escherichia coli. The fourth and fifth segments may comprise one domain which is homologous to the catalytic subunits of cAMP-dependent protein kinase, of calcium-dependent phosphorylase b kinase, and of certain oncogenic viral protein tyrosine kinases. The regulatory, amino-terminal half of cGMP-dependent protein kinase appears to be related to a family of smaller proteins that bind cAMP for diverse purposes, whereas the catalytic, carboxyl-terminal half is related to a family of protein kinases of varying specificity and varying sensitivity to regulators. These data suggest that ancestral gene splicing events may have been involved in the fusion of two families of proteins to generate the allosteric character of this chimeric enzyme.
cGMP 依赖性蛋白激酶的两个不同链内 cGMP 结合位点的研究。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Corbin,JD;Døskeland,SO
通讯作者：
Døskeland,SO
牛 II 型腺苷环状 3,5-磷酸依赖性蛋白激酶催化亚基的氨基酸序列。
影响因子：
2.9
作者：
Shoji,S;Ericsson,LH;Walsh,KA;Fischer,EH;Titani,K
通讯作者：
Titani,K
cGMP 依赖性蛋白激酶的自磷酸化。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Foster,JL;Guttmann,J;Rosen,OM
通讯作者：
Rosen,OM