High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents.
High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents.
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DOI：
10.1039/c7an01469d
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发表时间：
2018-01-15
期刊：
影响因子：
--
通讯作者：
Hage DS
中科院分区：
文献类型：
--
作者：
Zhang C ;Rodriguez E ;Bi C ;Zheng X ;Suresh D ;Suh K ;Li Z ;Elsebaei F ;Hage DS
在过去的几十年里，已经看到了许多使用生物相关结合剂的高性能分离方法的发展。高效液相色谱与这些结合剂的结合产生了一种称为高效亲和色谱（HPAC）的技术。本文综述了HPAC的一般原理及其相关技术，重点介绍了其在生物化合物和药物分析中的应用。将考虑这些方法的各种类型的结合剂，包括抗体、免疫球蛋白结合蛋白、适体、酶、凝集素、转运蛋白、脂质和碳水化合物。将讨论这些方法的格式将从分析物的直接检测到基于层析免疫测定的间接检测，以及基于分析物提取或耗尽、柱后检测和多柱系统的方案。在HPLC中使用生物制剂进行手性分离，以及使用HPAC作为筛选或研究生物相互作用的工具也将被考虑。我们将以不同的例子来说明这些方法及其在生物化学、临床化学和药物研究等领域的应用。
The last few decades have seen the development of many high performance separation methods that use biologically-related binding agents. The combination of HPLC with these binding agents results in a technique known as high performance affinity chromatography (HPAC). This review will discuss the general principles of HPAC and related techniques, with an emphasis on their use for the analysis of biological compounds and pharmaceutical agents. Various types of binding agents for these methods will be considered, including antibodies, immunoglobulin-binding proteins, aptamers, enzymes, lectins, transport proteins, lipids, and carbohydrates. Formats that will be discussed for these methods will range from the direct detection of an analyte to indirect detection based on chromatographic immunoassays, as well as schemes based on analyte extraction or depletion, post-column detection, and multi-column systems. The use of biological agents in HPLC for chiral separations will also be considered, along with the use of HPAC as a tool to screen or study biological interactions. Various examples will be presented to illustrate these approaches and their applications in fields such as biochemistry, clinical chemistry, and pharmaceutical research.
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影响因子：
4.2
作者：
Alwael, Hassan;Connolly, Damian;Paull, Brett
通讯作者：
Paull, Brett
影响因子：
56.9
作者：
ARMSTRONG, DW;WARD, TJ;BEESLEY, TE
通讯作者：
BEESLEY, TE
DOI：
10.1016/j.jchromb.2004.06.042
发表时间：
2004-10-05
影响因子：
3
作者：
Bergström, M;Nilsson, M;Ohlson, S
通讯作者：
Ohlson, S
影响因子：
4.1
作者：
Breton, D;Buret, D;Lafosse, A
通讯作者：
Lafosse, A
DOI：
10.1016/j.chroma.2017.03.056
发表时间：
2017-05-12
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Beeram S;Bi C;Zheng X;Hage DS
通讯作者：
Hage DS