Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway.
Novel recombinant R-spondin1 promotes hair regeneration by targeting the Wnt/β-catenin signaling pathway.
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DOI：
10.3724/abbs.2023112
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发表时间：
2023-07-20
影响因子：
3.7
通讯作者：
Gao C
中科院分区：
文献类型：
--
作者：
Chen Y;Lu Z;Feng J;Chen Z;Liu Z;Wang X;Yan H;Gao C
屋顶板特异性脊椎蛋白1（R-spondin 1，RSPO 1）是一种Wnt/β-catenin信号通路激活剂，与Wnt配体结合，刺激Wnt/β-catenin信号通路，这是毛发再生的关键。然而，目前尚不清楚重组RSPO 1（rRSPO 1）是否影响毛发再生。在此，我们用rRSPO 1处理C57 BL/6雄性小鼠，并研究背部皮肤中Wnt/β-catenin信号通路的表达和毛囊干细胞的活化。测定小鼠皮肤颜色评分和毛发覆盖面积以描述毛发生长，并对皮肤样品进行H&E染色、蛋白质印迹分析和免疫荧光染色以评价毛囊发育和Wnt/β-连环蛋白信号通路相关蛋白的表达。我们发现rRSPO 1激活小鼠毛囊干细胞（mHFSC）并加速毛发再生。rRSPO 1增加毛发覆盖面积、毛囊数量以及毛囊直径和长度。此外，rRSPO 1增强Wnt/β-catenin信号通路相关蛋白的活性和HFSC标志物的表达，以及mHFSC活力。这些结果表明，皮下注射rRSPO 1可以通过激活Wnt/β-catenin信号通路来改善毛囊发育，从而促进毛发再生。这项研究表明，rRSPO 1具有通过激活Wnt/β-catenin信号通路治疗脱发的潜力。
Roof plate-specific spondin 1 (R-spondin1, RSPO1) is a Wnt/β-catenin signaling pathway activator that binds with Wnt ligands to stimulate the Wnt/β-catenin signaling pathway, which is key to hair regeneration. However, it is not clear whether recombinant RSPO1 (rRSPO1) affects hair regeneration. Here, we treat C57BL/6 male mice with rRSPO1 and investigate the expression of the Wnt/β-catenin signaling pathway and the activation of hair follicle stem cells in the dorsal skin. The mouse skin color score and hair-covered area are determined to describe hair growth, and the skin samples are subjected to H&E staining, western blot analysis and immunofluorescence staining to evaluate hair follicle development and the expressions of Wnt/β-catenin signaling pathway-related proteins. We find that rRSPO1 activates mouse hair follicle stem cells (mHFSCs) and accelerates hair regeneration. rRSPO1 increases the hair-covered area, the number of hair follicles, and the hair follicle diameter and length. Moreover, rRSPO1 enhances the activity of Wnt/β-catenin signaling pathway-related proteins and the expressions of HFSC markers, as well as mHFSC viability. These results indicate that subcutaneous injection of rRSPO1 can improve hair follicle development by activating the Wnt/β-catenin signaling pathway, thereby promoting hair regeneration. This study demonstrates that rRSPO1 has the potential to treat hair loss by activating the Wnt/β-catenin signaling pathway.
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影响因子：
21.3
作者：
Flores A;Schell J;Krall AS;Jelinek D;Miranda M;Grigorian M;Braas D;White AC;Zhou JL;Graham NA;Graeber T;Seth P;Evseenko D;Coller HA;Rutter J;Christofk HR;Lowry WE
通讯作者：
Lowry WE
DOI：
10.1084/jem.20101559
发表时间：
2011-02-14
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Takashima S;Kadowaki M;Aoyama K;Koyama M;Oshima T;Tomizuka K;Akashi K;Teshima T
通讯作者：
Teshima T
影响因子：
64.5
作者：
Rabbani P;Takeo M;Chou W;Myung P;Bosenberg M;Chin L;Taketo MM;Ito M
通讯作者：
Ito M
影响因子：
6.5
作者：
Kwack, Mi H.;Kim, Moon K.;Sung, Young K.
通讯作者：
Sung, Young K.
影响因子：
64.8
作者：
Hao, Huai-Xiang;Xie, Yang;Cong, Feng
通讯作者：
Cong, Feng