New roles for the Arabidopsis TAO1 gene besides disease resistance
New roles for the Arabidopsis TAO1 gene besides disease resistance
复制标题
拟南芥TAO1基因除抗病外的新作用
DOI：
10.1134/s1021443715040196
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发表时间：
2015-06
影响因子：
1.4
通讯作者：
Hou S.
中科院分区：
文献类型：
--
作者：
Yang J.;Yan L.;Song Y.;Chai C.;Song L.;Guan L.;Hou S.
AvrB操作靶蛋白（Target of AvrB Operation, TAO1）是一种响应无毒基因B （AvrB）的TIR-NB-LRR抗病蛋白。然而，TAO1是否在拟南芥发育和/或非生物胁迫中发挥作用尚不清楚。在这里，我们发现TAO1的表达受到组织特异性的调节，并且TAO1蛋白定位于质膜和叶绿体。此外，我们发现三个陶1 T-DNA插入突变体没有明显的异常表型。然而，我们发现，乙烯前体ACC和生长素NAA上调了TAO1的表达，而乙烯抑制剂AgNO3和生长素抑制剂NPA下调了TAO1的表达，表明乙烯和生长素协同调节了TAO1的表达。我们还发现，与野生型相比，桃1-10过表达系的内源乙烯产量下降，而桃1-10 T-DNA突变系的内源乙烯产量增加，这表明桃1可能是乙烯信号的负调节因子。这些新特征揭示了韬1在拟南芥中独立于抗病作用的新作用。
Target of AvrB Operation (TAO1) is a TIR-NB-LRR disease resistance protein that responds to avirulence gene B (avrB). However, whether TAO1 plays a role in Arabidopsis thaliana development and/or abiotic stress is unknown. Here, we found that TAO1 expression is regulated in a tissue-specific manner, and that TAO1 protein is localized to both the plasma membrane and chloroplasts. Moreover, we showed that three tao1 T-DNA insertion mutants had no significant abnormal phenotype. However, we found that TAO1 expression was up-regulated by ethylene precursor ACC and auxin NAA, but down-regulated by ethylene inhibitor AgNO3 and auxin inhibitor NPA, suggesting that TAO1 is coordinately regulated by ethylene and auxin. We also found that endogenous ethylene production decreased in TAO1-overexpression lines, but increased in the tao1-10 T-DNA mutant line compared to the wild type, suggesting that TAO1 may be a negative regulator of ethylene signaling. These novel characteristics shed new light on the role(s) of TAO1 in Arabidopsis independent of its role in disease resistance.
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影响因子：
9.2
作者：
Wirthmueller, Lennart;Zhang, Yan;Parker, Jane E.
通讯作者：
Parker, Jane E.
影响因子：
56.9
作者：
BJ Staskawicz;FM Ausubel;BJ Baker;JG Ellis;JD Jones
通讯作者：
BJ Staskawicz;FM Ausubel;BJ Baker;JG Ellis;JD Jones
影响因子：
5.6
作者：
Bi D;Johnson KC;Zhu Z;Huang Y;Chen F;Zhang Y;Li X
通讯作者：
Li X
DOI：
10.1111/j.1365-313x.2007.03129.x
发表时间：
2007-07
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
T. Yuan;S. Fujioka;S. Takatsuto;S. Matsumoto;X. Gou;Kai He;S. Russell;Jia Li
通讯作者：
T. Yuan;S. Fujioka;S. Takatsuto;S. Matsumoto;X. Gou;Kai He;S. Russell;Jia Li
DOI：
10.1111/j.1365-313x.2006.02834.x
发表时间：
2006-09
期刊：
The Plant journal : for cell and molecular biology
影响因子：
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作者：
L. Michael Weaver;M. Swiderski;Yan Li;Jonathan D. G. Jones
通讯作者：
L. Michael Weaver;M. Swiderski;Yan Li;Jonathan D. G. Jones