Differential synaptic inputs to the cell body and proximal dendrites of preganglionic parasympathetic neurons in the rat conus medullaris.
Differential synaptic inputs to the cell body and proximal dendrites of preganglionic parasympathetic neurons in the rat conus medullaris.
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DOI：
10.1016/j.neuroscience.2008.09.017
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发表时间：
2008-12-02
期刊：
影响因子：
3.3
通讯作者：
Havton LA
中科院分区：
文献类型：
--
作者：
Persson S;Havton LA
节前副交感神经元（PPN）位于大鼠腰骶脊髓的中间外侧（IML）核中，有助于内脏盆腔器官的自主控制。 PPN 为源自脊髓的副交感神经传出信息提供最终的共同通路。我们检查了大鼠髓圆锥细胞体和 PPN 近端树突的突触输入类型和组织的详细超微结构。通过全身施用与辣根过氧化物酶缀合的霍乱毒素 B 亚基，对 PPN 进行逆行标记。我们展示了四种不同类型的突触纽扣与 PPN 体细胞和近端树突并置：S 型纽扣显示出清晰的球形囊泡； F型纽扣显示扁平的囊泡； DCV 型布顿显示出透明和致密核心囊泡的混合物； L 型纽扣很少见，但很大，表现出清晰的球状囊泡，并且仅在我们的样品中与 PPN 树突并置时遇到。与胞体相比，PPN 的近端树突覆盖的膜表面明显更高。与近端树突相比，PPN 胞体的抑制性突触影响明显更高，正如与胞体并置的假定抑制性 F 型按钮的比例较高以及近端树突每膜长度上 S 型按钮的频率较高所表明的那样。我们的研究表明，PPN 的突触输入源自多个不同的来源，并且在细胞膜表面上有差异地分布和整合。
Preganglionic parasympathetic neurons (PPNs) reside in the intermediolateral (IML) nucleus of the rat lumbosacral spinal cord and contribute to the autonomic control of visceral pelvic organs. PPNs provide the final common pathway for efferent parasympathetic information originating in the spinal cord. We examined the detailed ultrastructure of the type and organization of synaptic inputs to the cell body and proximal dendrites of PPNs in the rat conus medullaris. The PPNs were retrogradely labeled by a systemic administration of the B subunit of cholera toxin conjugated to horseradish peroxidase. We demonstrate four distinct types of synaptic boutons in apposition with PPN somata and proximal dendrites: S-type boutons show clear, spheroid vesicles; F-type boutons show flattened vesicles; DCV-type boutons show a mixture of clear and dense-cored vesicles; L-type boutons were rare, but large, exhibited clear spheroid vesicles, and were only encountered in apposition with the PPN dendrites in our sample. The membrane surface covered by apposed boutons was markedly higher for the proximal dendrites of PPNs, compared to their somata. The inhibitory synaptic influence was markedly higher over the PPN somata compared to their proximal dendrites, as suggested by the higher proportion of putative inhibitory F-type boutons in apposition with the soma and a higher frequency of S-type boutons per membrane length for the proximal dendrites. Our studies suggest that the synaptic input to PPNs originates from multiple distinct sources and is differentially distributed and integrated over the cell membrane surface.
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影响因子：
2.5
作者：
HANCOCK, MB;PEVETO, CA
通讯作者：
PEVETO, CA
影响因子：
2.5
作者：
Alvarez, FJ;Villalba, RM;Schneider, SP
通讯作者：
Schneider, SP
影响因子：
2.5
作者：
HAMILTON, MO;PAPKA, RE;MCNEILL, DL
通讯作者：
MCNEILL, DL
影响因子：
2.5
作者：
NADELHAFT, I;BOOTH, AM
通讯作者：
BOOTH, AM
DOI：
10.1007/bf01175559
发表时间：
1979-01-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
BROWN, HK;NOLAN, MF
通讯作者：
NOLAN, MF