Resistance to BmNPV via overexpression of an exogenous gene controlled by an inducible promoter and enhancer in transgenic silkworm, Bombyx mori.
Resistance to BmNPV via overexpression of an exogenous gene controlled by an inducible promoter and enhancer in transgenic silkworm, Bombyx mori.
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转基因家蚕中诱导型启动子和增强子控制的外源基因过表达对 BmNPV 的抗性
DOI：
10.1371/journal.pone.0041838
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Xia Q
中科院分区：
文献类型：
--
作者：
Jiang L;Cheng T;Zhao P;Yang Q;Wang G;Jin S;Lin P;Xiao Y;Xia Q
The hycu-ep32 gene of Hyphantria cunea NPV can inhibit Bombyx mori nucleopolyhedrovirus (BmNPV) multiplication in co-infected cells, but it is not known whether the overexpression of the hycu-ep32 gene has an antiviral effect in the silkworm, Bombyx mori. Thus, we constructed four transgenic vectors, which were under the control of the 39 K promoter of BmNPV (39 KP), Bombyx mori A4 promoter (A4P), hr3 enhancer of BmNPV combined with 39 KP, and hr3 combined with A4P. Transgenic lines were created via embryo microinjection using practical diapause silkworm. qPCR revealed that the expression level of hycu-ep32 could be induced effectively after BmNPV infection in transgenic lines where hycu-ep32 was controlled by hr3 combined with 39 KP (i.e., HEKG). After oral inoculation of BmNPV with 3 × 105 occlusion bodies per third instar, the mortality with HEKG-B was approximately 30% lower compared with the non-transgenic line. The economic characteristics of the transgenic lines remained unchanged. These results suggest that overexpression of an exogenous antiviral gene controlled by an inducible promoter and enhancer is a feasible method for breeding silkworms with a high antiviral capacity.
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影响因子：
5.4
作者：
RODEMS, SM;FRIESEN, PD
通讯作者：
FRIESEN, PD
影响因子：
3.7
作者：
GUARINO, LA;DONG, W
通讯作者：
DONG, W
影响因子：
3.8
作者：
KOOL, M;VOETEN, JTM;VLAK, JM
通讯作者：
VLAK, JM
DOI：
10.1073/pnas.91.23.11212
发表时间：
1994-11-08
影响因子：
11.1
作者：
KOOL, M;AHRENS, CH;VLAK, JM
通讯作者：
VLAK, JM
影响因子：
5.4
作者：
MORRIS, TD;MILLER, LK
通讯作者：
MILLER, LK