Microwave dielectric heating of drops in microfluidic devices.
Microwave dielectric heating of drops in microfluidic devices.
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DOI：
10.1039/b822357b
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发表时间：
2009-06-21
期刊：
影响因子：
6.1
通讯作者：
Westervelt RM
中科院分区：
文献类型：
--
作者：
Issadore D;Humphry KJ;Brown KA;Sandberg L;Weitz DA;Westervelt RM
提出了一种用微波介质加热局部快速加热微流控装置中的水滴的技术。水比聚合物、玻璃和油更有效地吸收微波功率，因为它的永久分子偶极矩在GHz频率上有很大的介电损耗。系统的相关热容是单个热隔离的皮升水滴，这可以实现非常快的热循环。我们演示了一种微流体装置中的微波介电加热，该装置集成了流聚焦液滴制造器、液滴分离器和金属电极，可以从廉价的、市售的3.0 GHz源和放大器本地提供微波功率。通过观察悬浮在水滴中的硒化镉纳米晶体的温度依赖荧光强度来测量水滴的温度。我们展示了特征加热时间短至15毫秒，稳态温度高达30°C以上的微流体装置的基础温度。许多常见的生物和化学应用需要快速和局部控制温度，例如DNA的PCR扩增，并且可以从这项新技术中受益。
We present a technique to locally and rapidly heat water drops in microfluidic devices with microwave dielectric heating. Water absorbs microwave power more efficiently than polymers, glass, and oils due to its permanent molecular dipole moment that has a large dielectric loss at GHz frequencies. The relevant heat capacity of the system is a single thermally isolated picoliter drop of water and this enables very fast thermal cycling. We demonstrate microwave dielectric heating in a microfluidic device that integrates a flow-focusing drop maker, drop splitters, and metal electrodes to locally deliver microwave power from an inexpensive, commercially available 3.0 GHz source and amplifier. The temperature of the drops is measured by observing the temperature dependent fluorescence intensity of cadmium selenide nanocrystals suspended in the water drops. We demonstrate characteristic heating times as short as 15 ms to steady-state temperatures as large as 30°C above the base temperature of the microfluidic device. Many common biological and chemical applications require rapid and local control of temperature, such as PCR amplification of DNA, and can benefit from this new technique.
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影响因子：
6.1
作者：
Hunt, Thomas P.;Issadore, David;Westervelt, R. M.
通讯作者：
Westervelt, R. M.
DOI：
10.6028/jres.112.015
发表时间：
2007-07
影响因子：
1.5
作者：
Geist J;Shah JJ;Rao MV;Gaitan M
通讯作者：
Gaitan M
影响因子：
6.1
作者：
Martinez AW;Phillips ST;Wiley BJ;Gupta M;Whitesides GM
通讯作者：
Whitesides GM
影响因子：
1.6
作者：
NAKANO, H;MATSUDA, K;YAMANE, T
通讯作者：
YAMANE, T
影响因子：
7.4
作者：
Oda, RP;Strausbauch, MA;Landers, JP
通讯作者：
Landers, JP