Localisation pattern of Foxp3+ regulatory T cells is associated with clinical behaviour in gastric cancer.
Localisation pattern of Foxp3+ regulatory T cells is associated with clinical behaviour in gastric cancer.
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DOI：
10.1038/sj.bjc.6604149
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发表时间：
2008-01-15
影响因子：
8.8
通讯作者：
Fujii, H.
中科院分区：
文献类型：
--
作者：
Mizukami, Y.;Kono, K.;Kawaguchi, Y.;Akaike, H.;Kamimura, K.;Sugai, H.;Fujii, H.
据报道，在肿瘤宿主的肿瘤浸润淋巴细胞中，调节性T细胞（T regs）的数量增加。叉头/翼螺旋转录因子p3 （Foxp3）被认为是最可靠的T regs标记。在本研究中，我们通过免疫组织化学研究了Foxp3+细胞在胃癌（n=80）中的流行程度和定位模式，以及与胃癌患者临床预后的关系。用抗Foxp3单抗进行免疫组化染色，对Foxp3+细胞进行半定量。我们根据Foxp3+细胞群的中位数大小将所有病例分为两组：Foxp3+高组（n=40）和Foxp3+低组（n=40）。此外，根据Foxp3+细胞在肿瘤中积聚的定位模式，我们将所有病例分为三组：肿瘤周围组（n=30），弥漫性组（n=40）和滤泡组（n=10）。因此，尽管IV期Foxp3+细胞数量明显多于I期（P<0.05），但Foxp3+细胞数量高、低患者的生存期差异无统计学意义。然而，Foxp3+细胞弥漫型患者的生存率明显低于肿瘤周围型患者。综上所述，Foxp3+细胞的定位模式，而不是群体大小，与患者的生存有显著关系。
It has been reported that the population of regulatory T cells (T regs) is increased in tumour-infiltrating lymphocytes in cancer-bearing hosts. Recently, forkhead/winged helix transcription factor p3, Foxp3, is thought to be the most reliable marker of T regs. In the present study, we investigated the prevalence and localisation pattern of Foxp3+ cells in gastric cancer (n=80) by immunohistochemistry, in relation to the clinical outcome of gastric cancer patients. Immunohistochemical staining was performed with anti-Foxp3 mAb, and Foxp3+ cells were semiquantified. We divided all cases into two groups: Foxp3+-high (n=40) and Foxp3+-low (n=40) groups, by the median size of the population of Foxp3+ cells. Furthermore, in terms of the localisation pattern of accumulating Foxp3+ cells in tumours, we classified all cases into three groups: a peri-tumour group (n=30), a diffuse group (n=40), and a follicular group (n=10). As a result, although the populations of Foxp3+ cells in stage IV were significantly larger than those in stage I (P<0.05), there was no significant difference in survival between the patients with high and low population levels of Foxp3+ cells. However, survival in patients with a diffuse pattern of Foxp3+ cells was significantly poorer than in those with a peri-tumoral pattern. In conclusion, the localisation pattern, but not the population size, of Foxp3+ cells was significantly related to patient survival.
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DOI：
10.1084/jem.193.11.1303
发表时间：
2001-06-04
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Dieckmann D;Plottner H;Berchtold S;Berger T;Schuler G
通讯作者：
Schuler G
影响因子：
4.4
作者：
Misra, N;Bayry, J;Kaveri, SV
通讯作者：
Kaveri, SV
影响因子：
32.4
作者：
Fontenot, JD;Rasmussen, JP;Rudensky, AY
通讯作者：
Rudensky, AY
影响因子：
4.4
作者：
Smyth, MJ;Teng, MWL;Hayakawa, Y
通讯作者：
Hayakawa, Y
影响因子：
11.5
作者：
Badoual, C;Hans, S;Tartour, E
通讯作者：
Tartour, E