Synthetic torpor protects rats from exposure to accelerated heavy ions.
Synthetic torpor protects rats from exposure to accelerated heavy ions.
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DOI：
10.1038/s41598-022-20382-6
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发表时间：
2022-09-30
影响因子：
4.6
通讯作者：
Tinganelli, Walter
中科院分区：
文献类型：
--
作者：
Puspitasari, Anggraeini;Squarcio, Fabio;Quartieri, Martina;Totis, Cristina;Hitrec, Timna;Takahashi, Akihisa;Yoshida, Yukari;Hanamura, Kenji;Yako, Tomoko;Cerri, Matteo;Simoniello, Palma;Durante, Marco;Tinganelli, Walter
冬眠或麻痹被认为是保护宇航员免受含有高能重离子的空间辐射有害影响的一种可能的工具。我们用加速碳离子（C-ions）全身照射（TBI）后立即腹腔注射5′-单磷酸腺苷（5′-AMP）并在低温（+ 16 °C）室中保持6 h，诱导大鼠合成性麻痹。与注射生理盐水的动物相比，5′-AMP治疗结合低环境温度降低了皮肤温度，并增加了8戈伊C离子照射后的存活率。脑、肝和肺的组织学分析显示，2戈伊TBI后5′-AMP治疗减少了活化的小胶质细胞、脑中的Iba 1阳性细胞、肝中的凋亡细胞和肺损伤，表明合成性麻痹使组织免受高能离子辐射的影响。与对照组相比，5′-AMP与低氧或低温环境联合照射大鼠视网膜色素上皮细胞6小时后，可延迟DNA修复并抑制辐射诱导的有丝分裂灾难。我们的结论是，合成麻木保护动物免受宇宙射线模拟辐射和机制涉及低温和缺氧。
Hibernation or torpor is considered a possible tool to protect astronauts from the deleterious effects of space radiation that contains high-energy heavy ions. We induced synthetic torpor in rats by injecting adenosine 5′-monophosphate monohydrate (5′-AMP) i.p. and maintaining in low ambient temperature room (+ 16 °C) for 6 h immediately after total body irradiation (TBI) with accelerated carbon ions (C-ions). The 5′-AMP treatment in combination with low ambient temperature reduced skin temperature and increased survival following 8 Gy C-ion irradiation compared to saline-injected animals. Analysis of the histology of the brain, liver and lungs showed that 5′-AMP treatment following 2 Gy TBI reduced activated microglia, Iba1 positive cells in the brain, apoptotic cells in the liver, and damage to the lungs, suggesting that synthetic torpor spares tissues from energetic ion radiation. The application of 5′-AMP in combination with either hypoxia or low temperature environment for six hours following irradiation of rat retinal pigment epithelial cells delays DNA repair and suppresses the radiation-induced mitotic catastrophe compared to control cells. We conclude that synthetic torpor protects animals from cosmic ray-simulated radiation and the mechanism involves both hypothermia and hypoxia.
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影响因子：
4.6
作者：
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通讯作者：
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