A Novel Structure Harboring bla(CTX-M-27) on IncF Plasmids in Escherichia coli Isolated from Swine in China.
A Novel Structure Harboring bla(CTX-M-27) on IncF Plasmids in Escherichia coli Isolated from Swine in China.
复制标题
中国猪源大肠埃希菌INCF质粒上含有BLA(CTX-M-27)的新结构。
DOI：
10.3390/antibiotics10040387
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发表时间：
2021-04-04
期刊：
影响因子：
--
通讯作者：
Jiang HX
中科院分区：
文献类型：
--
作者：
Zhang Y;Sun YH;Wang JY;Chang MX;Zhao QY;Jiang HX
本研究旨在探讨中国猪场产BLACTX-M-27菌株的流行情况及传播机制。2013至2016年间，从两个养殖场共收集到333株大肠杆菌。共分离到32株CTX-M-27阳性的大肠埃希菌，且均为多重耐药。脉冲场凝胶电泳(PFGE)和多位点序列分型(MLST)图谱显示携带blaCTX-M-27的菌株类型广泛，以ST10序列类型为主。接合、复制子分型、S1-PFGE和杂交实验证实，32株CTX-M-27阳性菌株中有28株在F18：A-：B10(16)和F24：A-：B1(12)上携带blaCTX-M-27基因，24株菌株的blaCTX-M-27基因是通过大小为40~155kb的质粒传递的。与blaCTX-M-27-质粒的比较分析表明，在F18：A-：B10(12个菌株)中插入了一个复合区(8个菌株)和一个含有blaCTX-M-27的新结构，从而破坏了F24：A-：B1的tra-TRB区。这种新的结构提高了blaCTX-M-27基因在大肠杆菌中的稳定性。这项研究表明，随着时间的推移，传播blaCTX-M-27的主要媒介已经多样化，有必要采取控制策略来限制blaCTX-M-27在家畜中的传播。
The aim of this study was to elucidate the prevalence of blaCTX-M-27-producing Escherichia coli and transmission mechanisms of blaCTX-M-27 from swine farms in China. A total of 333 E. coli isolates were collected from two farms from 2013 to 2016. Thirty-two CTX-M-27-positive E. coli were obtained, and all were multidrug-resistant. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) profiles indicated a wide range of strain types that carried blaCTX-M-27, and the sequence type ST10 predominated. Conjugation, replicon typing, S1-PFGE and hybridization experiments confirmed that 28 out of 32 CTX-M-27 positive isolates carried blaCTX-M-27 genes on plasmids F18:A-:B10 (16) and F24:A-:B1 (12).The blaCTX-M-27 genes for 24 isolates were transmitted by plasmids with sizes ranging from 40 to 155 kb. A comparative analysis with blaCTX-M-27-plasmids indicated that the tra-trb region of F24:A-:B1 plasmids was destroyed by insertion of a complex region (eight isolates) and a novel structure containing blaCTX-M-27 in the F18:A-:B10 plasmids (12 isolates). The novel structure increased the stability of the blaCTX-M-27 gene in E. coli. This study indicated that the predominant vehicle for blaCTX-M-27 transmission has diversified over time and that control strategies to limit blaCTX-M-27 transmission in farm animals are necessary.
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影响因子：
3.3
作者：
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