Endophytic fungi associated with Monarda citriodora, an aromatic and medicinal plant and their biocontrol potential.
Endophytic fungi associated with Monarda citriodora, an aromatic and medicinal plant and their biocontrol potential.
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DOI：
10.1080/13880209.2017.1309054
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发表时间：
2017-12
影响因子：
3.8
通讯作者：
Pull S
中科院分区：
文献类型：
--
作者：
Katoch M;Pull S
背景：粮食及农业组织估计，每年由于植物病害造成粮食作物的大量损失。尽管杀真菌剂广泛用于植物病害的管理，但它们昂贵且对环境和人类健康有害。另外，生物防治是克服植物病害影响和维持农业的安全方法。学名Monarda citriodora Cerv.前滞后（唇形科/唇形科）以其抗真菌特性而闻名，因此被选择用于研究。目的：分离毛白杨内生真菌。Citriodora和评估其生物防治潜力。材料与方法：采用ITS-5.8SrDNA序列测定方法对分离的内生菌进行鉴定。它们的生物防治潜力进行了评估，使用不同的拮抗试验对主要的植物病原体。结果如下：共分离到11属28株内生菌，其中约82%的内生菌对植物病原菌具有生防潜力。MC-2L（尖孢镰刀菌）、MC-14F（尖孢镰刀菌）、MC-14L（尖孢镰刀菌）、MC-14F（尖孢镰刀菌）。oxysporum）、MC-22 F（F. oxysporum）和MC-25 F（F. redolens）对所有测试的病原体显示出显著的拮抗活性。有趣的是，MC-10 L（Muscodor yucatanensis）完全抑制核盘菌的生长，辣椒炭疽菌（Colletotrichum capsici）、黄曲霉（Aspergillus flavus）和A. MC-8 L（A.在熏蒸试验中，MC-9L（Penicilliumcommune）和MC-9L（Penicilliumcommune）完全抑制了核盘菌的生长。结论：内生菌MC-2 L、MC-14 F、MC-22 F和MC-25 F可有效地防治多种植物病原菌，而MC-10 L、MC-8 L和MC-9 L可有效地防治特定病原菌。其次，内生菌在不同的测定中表现出不同程度的拮抗作用，代表了化学多样性，不仅作为有前途的生物防治剂，而且作为防御和生物活性代谢产物的资源。
Context: The Food and Agriculture Organization has estimated that every year considerable losses of the food crops occur due to plant diseases. Although fungicides are extensively used for management of plant diseases, they are expensive and hazardous to the environment and human health. Alternatively, biological control is the safe way to overcome the effects of plant diseases and to sustain agriculture. Since Monarda citriodora Cerv. ex Lag. (Lamiaceae/Labiatae) is known for its antifungal properties, it was chosen for the study. Objective: The isolation of endophytic fungi from M. citriodora and assessing their biocontrol potential. Material and methods: The isolated endophytes were characterized using ITS-5.8 S rDNA sequencing. Their biocontrol potential was assessed using different antagonistic assays against major plant pathogens. Results: Twenty-eight endophytes representing 11 genera were isolated, of which, around 82% endophytes showed biocontrol potential against plant pathogens. MC-2 L (Fusarium oxysporum), MC-14 F (F. oxysporum), MC-22 F (F. oxysporum) and MC-25 F (F. redolens) displayed significant antagonistic activity against all the tested pathogens. Interestingly, MC-10 L (Muscodor yucatanensis) completely inhibited the growth of Sclerotinia sp., Colletotrichum capsici, Aspergillus flavus and A. fumigatus in dual culture assay, whereas MC-8 L (A. oryzae) and MC-9 L (Penicillium commune) completely inhibited the growth of the Sclerotinia sp. in fumigation assay. Conclusions: Endophytes MC-2 L, MC-14 F, MC-22 F and MC-25 F could effectively be used to control broad range of phytopathogens, while MC-10 L, MC-8 L and MC-9 L could be used to control specific pathogens. Secondly, endophytes showing varying degrees of antagonism in different assays represented the chemo-diversity not only as promising biocontrol agents but also as a resource of defensive and bioactive metabolites.
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DOI：
10.1073/pnas.2533483100
发表时间：
2003-12-23
影响因子：
11.1
作者：
Arnold, AE;Mejía, LC;Herre, EA
通讯作者：
Herre, EA
影响因子：
3.2
作者：
Benhamou, N;Gagné, S;Dehbi, L
通讯作者：
Dehbi, L
影响因子：
3.4
作者：
Chamberlain, K;Crawford, DL
通讯作者：
Crawford, DL
DOI：
10.1016/s0007-1536(71)80077-3
发表时间：
1971-01-01
期刊：
TRANSACTIONS OF THE BRITISH MYCOLOGICAL SOCIETY
影响因子：
--
作者：
DENNIS, C;WEBSTER, J
通讯作者：
WEBSTER, J
影响因子：
3.7
作者：
Ganley, Rebecca J.;Sniezko, Richard A.;Newcombe, George
通讯作者：
Newcombe, George