Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues.
Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues.
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DOI：
10.1016/j.biomaterials.2011.04.008
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发表时间：
2011-08
期刊：
影响因子：
14
通讯作者：
Han, Bumsoo
中科院分区：
文献类型：
--
作者：
Teo, Ka Yaw;DeHoyos, Tenok O.;Dutton, J. Craig;Grinnell, Frederick;Han, Bumsoo
成功的工程组织冷冻保存的两个最重要的挑战是保存组织功能和控制高度依赖组织类型的保存结果。为了应对这些挑战，必须了解冷冻诱导的细胞-流体-基质相互作用，这些相互作用决定了解冻后细胞的活性和细胞外基质(ECM)的微观结构。然而，目前对这种组织水平的生物物理相互作用的了解仍然有限。本研究以真皮替代物为模型，研究了冷冻诱导的细胞-流体-基质相互作用及其对内皮细胞和细胞外基质微结构的影响。将人真皮成纤维细胞种植在不同细胞种植密度和胶原浓度的I型胶原基质中，构建成纤维细胞真皮替代物。虽然这些真皮的等价物经历了相同的冷冻/解冻条件，但它们在冷冻过程中的时空变形、解冻后的ECM微观结构和细胞水平的冷冻反应被表征。结果表明，各试验组的冻结变形程度和变形特征有显著差异，ECM组织越致密的ETS变形越大。变形的大小与解冻后的ECM结构有很好的相关性，说明冻结引起的变形是解冻后ECM结构的良好指标。细胞损伤的程度在各实验组之间也有显著差异，这取决于冷冻引起的ETS变形的程度和初始细胞骨架的组织。这些结果表明，由于冷冻引起的变形和热损伤，细胞受到了机械损伤。这些发现提供了对依赖于组织类型的冷冻保存结果的洞察，并有助于根据预先开发的冷冻保存方案为新类型的组织设计和修改冷冻保存方案。
The two most significant challenges for successful cryopreservation of engineered tissues (ETs) are preserving tissue functionality and controlling highly tissue-type dependent preservation outcomes. In order to address these challenges, freezing-induced cell-fluid-matrix interactions should be understood, which determine the post-thaw cell viability and extracellular matrix (ECM) microstructure. However, the current understanding of this tissue-level biophysical interaction is still limited. In this study, freezing-induced cell-fluid-matrix interactions and their impact on the cells and ECM microstructure of ETs were investigated using dermal equivalents as a model ET. The dermal equivalents were constructed by seeding human dermal fibroblasts in type I collagen matrices with varying cell seeding density and collagen concentration. While these dermal equivalents underwent an identical freeze/thaw condition, their spatiotemporal deformation during freezing, post-thaw ECM microstructure, and cellular level cryoresponse were characterized. The results showed that the extent and characteristics of freezing-induced deformation were significantly different among the experimental groups, and the ETs with denser ECM microstructure experienced a larger deformation. The magnitude of the deformation was well correlated to the post-thaw ECM structure, suggesting that the freezing-induced deformation is a good indicator of post-thaw ECM structure. A significant difference in the extent of cellular injury was also noted among the experimental groups, and it depended on the extent of freezing-induced deformation of the ETs and the initial cytoskeleton organization. These results suggest that the cells have been subjected to mechanical insult due to the freezing-induced deformation as well as thermal insult. These findings provide insight on tissue-type dependent cryopreservation outcomes, and can help to design and modify cryopreservation protocols for new types of tissues from a pre-developed cryopreservation protocol.
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DOI：
10.1002/jbm.b.30757
发表时间：
2007-08-01
影响因子：
3.4
作者：
Chen, Meng-Yi;Sun, Yulong;Amadio, Peter C.
通讯作者：
Amadio, Peter C.
影响因子：
--
作者：
Devireddy, RV;Neidert, MR;Tranquillo, RT
通讯作者：
Tranquillo, RT
影响因子：
4.1
作者：
Hakkinen, Kirsi M.;Harunaga, Jill S.;Yamada, Kenneth M.
通讯作者：
Yamada, Kenneth M.
影响因子：
2.7
作者：
Gerson, Cindy J.;Goldstein, Steven;Heacox, Albert E.
通讯作者：
Heacox, Albert E.
影响因子：
4.8
作者：
Berthiaume, F;Moghe, PV;Yarmush, ML
通讯作者：
Yarmush, ML