Label-free optical detection of biomolecular translocation through nanopore arrays.
Label-free optical detection of biomolecular translocation through nanopore arrays.
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DOI：
10.1021/nn504551d
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发表时间：
2014-10-28
期刊：
影响因子：
17.1
通讯作者：
Wanunu, Meni
中科院分区：
文献类型：
--
作者：
Ivankin, Andrey;Henley, Robert Y.;Larkin, Joseph;Carson, Spencer;Toscano, Michael L.;Wanunu, Meni
近年来，纳米孔已经成为非常有前途的单分子传感器，因为它们能够以无标记的方式检测亚飞摩尔水平的生物分子。开发高通量的基于纳米孔的生物传感器需要纳米孔测量的多路复用。然而，电检测带来了挑战，因为每个纳米孔电路必须是电独立的，这需要复杂的纳米流体和嵌入式电极。在这里，我们提出了一种光学方法，用于同时测量跨固态纳米孔阵列的离子电流，不需要额外的制造步骤。进行原理验证实验，其显示使用孔阵列同时光学检测和表征ssDNA和dsDNA。通过与电测量的比较，我们表明，光学测量是能够访问等效的跨膜电流信息。
In recent years, nanopores have emerged as exceptionally promising single-molecule sensors due to their ability to detect biomolecules at subfemtomole levels in a label-free manner. Development of a high-throughput nanopore-based biosensor requires multiplexing of nanopore measurements. Electrical detection, however, poses a challenge, as each nanopore circuit must be electrically independent, which requires complex nanofluidics and embedded electrodes. Here, we present an optical method for simultaneous measurements of the ionic current across an array of solid-state nanopores, requiring no additional fabrication steps. Proof-of-principle experiments are conducted that show simultaneous optical detection and characterization of ssDNA and dsDNA using an array of pores. Through a comparison with electrical measurements, we show that optical measurements are capable of accessing equivalent transmembrane current information.
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