Fabrication and characterization of nanopores with insulated transverse nanoelectrodes for DNA sensing in salt solution.
Fabrication and characterization of nanopores with insulated transverse nanoelectrodes for DNA sensing in salt solution.
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DOI：
10.1002/elps.201200350
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发表时间：
2012-12
期刊：
影响因子：
2.9
通讯作者：
Drndic, Marija
中科院分区：
文献类型：
--
作者：
Healy, Ken;Ray, Vishva;Willis, Lauren J.;Peterman, Neil;Bartel, John;Drndic, Marija
我们报道了在低电容氮化硅薄膜芯片中与纳米孔对齐的绝缘纳米电极的制造、模拟和表征。我们正在探索这些装置，用于横向感应DNA分子，因为它们是以线性方式通过纳米孔的电泳驱动。虽然我们目前正在使用相对较大的纳米孔（直径6-12纳米）来演示DNA的横向检测，但我们的最终目标是将尺寸减小到足以分解单个核苷酸碱基，从而在DNA通过孔时进行测序。我们提出了模拟和实验来研究绝缘这些电极的影响，这对定位传感区域很重要。我们测试纳米电极或绝缘的存在是否会影响流过纳米孔的离子电流的稳定性，或DNA易位的特性。最后，我们总结了常见的器件故障和在制造和实验过程中遇到的挑战，探讨了这些故障的原因，并提出了未来如何克服这些故障的建议。
We report on the fabrication, simulation, and characterization of insulated nanoelectrodes aligned with nanopores in low-capacitance silicon nitride membrane chips. We are exploring these devices for the transverse sensing of DNA molecules as they are electrophoretically driven through the nanopore in a linear fashion. While we are currently working with relatively large nanopores (6–12 nm in diameter) to demonstrate the transverse detection of DNA, our ultimate goal is to reduce the size sufficiently to resolve individual nucleotide bases, thus sequencing DNA as it passes through the pore. We present simulations and experiments that study the impact of insulating these electrodes, which is important to localize the sensing region. We test whether the presence of nanoelectrodes or insulation affects the stability of the ionic current flowing through the nanopore, or the characteristics of DNA translocation. Finally, we summarize the common device failures and challenges encountered during fabrication and experiments, explore the causes of these failures, and make suggestions on how to overcome them in the future.
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影响因子：
3.7
作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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