Ovarian cycling and reproductive state shape the vaginal microbiota in wild baboons.
Ovarian cycling and reproductive state shape the vaginal microbiota in wild baboons.
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卵巢循环和生殖状态塑造了野生狒狒的阴道菌群。
DOI：
10.1186/s40168-017-0228-z
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发表时间：
2017-01-19
期刊：
影响因子：
15.5
通讯作者：
Archie EA
中科院分区：
文献类型：
--
作者：
Miller EA;Livermore JA;Alberts SC;Tung J;Archie EA
阴道微生物组是细菌-哺乳动物共生的重要场所。这种共生关系目前最适合人类，其中乳酸杆菌占主导地位的微生物群落，并可能有助于保护妇女免受传染病。然而，迄今为止，乳酸杆菌并没有在任何其他哺乳动物的阴道微生物群中占主导地位，这引发了关于塑造非人类哺乳动物阴道微生物群的力量的关键问题。我们使用细菌16 S rRNA基因的Illumina测序来研究48只狒狒（Papio cynocephalus）阴道微生物群分类组成的变化，这些狒狒是肯尼亚一个经过充分研究的野生种群的成员。与之前的研究类似，我们发现狒狒阴道微生物群并不以乳酸杆菌为主。尽管存在这种差异，但与人类相似，生殖状态是狒狒阴道微生物群的主要预测因素，怀孕，产后闭经和卵巢周期解释了18%的群落组成方差。此外，在周期性的女性，一个惊人的39%的差异在社区组成的解释卵巢周期阶段，特别是独特的微生物群落排卵。排卵期女性表现出最高的相对丰度的乳酸产生菌相比，任何其他阶段，平均相对丰度为44%。在较小程度上，性行为，特别是共享性伴侣的历史，也预测了狒狒之间的阴道微生物相似性。尽管它们的优势微生物存在显着差异，但人类和狒狒阴道微生物群在生殖状态，卵巢周期阶段和性行为方面都表现出深刻的组成变化。我们发现在排卵期间成分发生了重大变化，这可能对疾病风险和受孕成功有影响。这些发现强调了未来研究的必要性，以解释生殖状态的细微差异，特别是卵巢周期各个阶段之间的差异。总的来说，我们的工作有助于对解释哺乳动物阴道微生物组个体内和个体间变异的力量的理解，特别强调其在宿主健康和疾病风险中的作用。本文的在线版本（doi：10.1186/s40168-017-0228-z）包含补充材料，可供授权用户使用。
The vaginal microbiome is an important site of bacterial-mammalian symbiosis. This symbiosis is currently best characterized for humans, where lactobacilli dominate the microbial community and may help defend women against infectious disease. However, lactobacilli do not dominate the vaginal microbiota of any other mammal studied to date, raising key questions about the forces that shape the vaginal microbiome in non-human mammals. We used Illumina sequencing of the bacterial 16S rRNA gene to investigate variation in the taxonomic composition of the vaginal microbiota in 48 baboons (Papio cynocephalus), members of a well-studied wild population in Kenya. Similar to prior studies, we found that the baboon vaginal microbiota was not dominated by lactobacilli. Despite this difference, and similar to humans, reproductive state was the dominant predictor of baboon vaginal microbiota, with pregnancy, postpartum amenorrhea, and ovarian cycling explaining 18% of the variance in community composition. Furthermore, among cycling females, a striking 39% of variance in community composition was explained by ovarian cycle phase, with an especially distinctive microbial community around ovulation. Periovulatory females exhibited the highest relative abundance of lactic acid-producing bacteria compared to any other phase, with a mean relative abundance of 44%. To a lesser extent, sexual behavior, especially a history of shared sexual partners, also predicted vaginal microbial similarity between baboons. Despite striking differences in their dominant microbes, both human and baboon vaginal microbiota exhibit profound changes in composition in response to reproductive state, ovarian cycle phase, and sexual behavior. We found major shifts in composition during ovulation, which may have implications for disease risk and conception success. These findings highlight the need for future studies to account for fine-scale differences in reproductive state, particularly differences between the various phases of the ovarian cycle. Overall, our work contributes to an emerging understanding of the forces that explain intra- and inter-individual variation in the mammalian vaginal microbiome, with particular emphasis on its role in host health and disease risk. The online version of this article (doi:10.1186/s40168-017-0228-z) contains supplementary material, which is available to authorized users.
影响因子：
0.7
作者：
Gravett MG;Jin L;Pavlova SI;Tao L
通讯作者：
Tao L