Lactobacillus and Pediococcus species richness and relative abundance in the vagina of rhesus monkeys (Macaca mulatta).
Lactobacillus and Pediococcus species richness and relative abundance in the vagina of rhesus monkeys (Macaca mulatta).
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DOI：
10.1111/j.1600-0684.2012.00537.x
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发表时间：
2012-06
影响因子：
0.7
通讯作者：
Tao L
中科院分区：
文献类型：
--
作者：
Gravett MG;Jin L;Pavlova SI;Tao L
恒河猴是研究人类阴道健康的重要动物模型，乳酸菌在其中起着重要的作用。然而，猕猴阴道乳酸菌的物种丰富度和相对丰度在很大程度上是未知的。阴道拭子标本取自200只生殖年龄雌性恒河猴。在Rogosa琼脂平板培养后，分离出代表不同形态类型的单个菌落，并对其进行全细胞蛋白图谱、物种特异性聚合酶链式反应和16S rRNA基因序列分析。共鉴定出乳杆菌17种510株，酸链球菌1株。最丰富的物种是路氏乳杆菌，它定植在86%的猴子的阴道里。约翰逊氏菌是第二多的物种，在36%的猴子中定居。大多数猴子被多种乳杆菌定植。恒河猴的阴道经常被多种乳杆菌定植，其中以路氏乳杆菌为主。
The rhesus monkey is an important animal model to study human vaginal health to which lactic acid bacteria play a significant role. However, the vaginal lactic acid bacterial species richness and relative abundance in rhesus monkeys is largely unknown. Vaginal swab samples were aseptically obtained from 200 reproductive aged female rhesus monkeys. Following Rogosa agar plating, single bacterial colonies representing different morphotypes were isolated and analyzed for whole-cell protein profile, species-specifc PCR, and 16S rRNA gene sequence. A total of 510 Lactobacillus strains of 17 species and one Pediococcus acidilactici were identified. The most abundant species was L. reuteri, which colonized the vaginas of 86% monkeys. L. johnsonii was the second most abundant species, which colonized 36% of monkeys. The majority of monkeys were colonized by multiple Lactobacillus species. The vaginas of rhesus monkeys are frequently colonized by multiple Lactobacillus species, dominated by L. reuteri.
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影响因子：
4
作者：
Jin, L.;Tao, L.;Wawer, M.
通讯作者：
Wawer, M.
影响因子：
3.2
作者：
Kant, Ravi;Paulin, Lars;Palva, Airi
通讯作者：
Palva, Airi
影响因子：
6.4
作者：
Hawes, SE;Hillier, SL;Holmes, KK
通讯作者：
Holmes, KK
影响因子：
0.7
作者：
Jin L;Hinde K;Tao L
通讯作者：
Tao L
影响因子：
4
作者：
Pavlova, SI;Kilic, AO;Tao, L
通讯作者：
Tao, L