Raman Spectroscopy detects changes in Bone Mineral Quality and Collagen Cross-linkage in Staphylococcus Infected Human Bone.
Raman Spectroscopy detects changes in Bone Mineral Quality and Collagen Cross-linkage in Staphylococcus Infected Human Bone.
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DOI：
10.1038/s41598-018-27752-z
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发表时间：
2018-06-20
影响因子：
4.6
通讯作者：
Dutta J
中科院分区：
文献类型：
--
作者：
Khalid M;Bora T;Ghaithi AA;Thukral S;Dutta J
骨髓炎的诊断提出了一个艰巨的挑战。缺乏特异性的临床体征和诊断测试，可以诊断骨髓炎在早期阶段有助于这一困难。如果不及早诊断，这种疾病就很难根除，并可能导致威胁肢体和潜在危及生命的并发症。金黄色葡萄球菌是引起骨髓炎最常见的微生物。拉曼光谱提供了关于分子振动的信息，这些信息可能被利用作为特定病理条件下细胞变化的光谱特征。在这项研究中，我们描述了一种技术，使用拉曼光谱，可能被用来诊断葡萄球菌骨髓炎。将人骨样品与金黄色葡萄球菌（S. aureus），然后使用拉曼光谱监测细菌生长对骨质量的影响。与对照组相比，在感染骨中观察到骨矿物质质量和结晶度的大幅下降。S.还发现金黄色葡萄球菌感染改变胶原交联。我们的研究表明，特定的光谱特征是目前的原因，以及葡萄球菌骨髓炎的影响，开辟了一个有用的诊断模式，这种情况的早期和快速诊断的可能性。
Diagnosis of osteomyelitis presents a formidable challenge. Lack of pathognomonic clinical sign(s) and diagnostic tests that can diagnose osteomyelitis at an early stage contribute to this difficulty. If the diagnosis is not made early, the disease becomes very difficult to eradicate and can lead to limb threatening and potentially life-threatening complications. Staphylococcus aureus is the most common organism causing osteomyelitis. Raman Spectroscopy provides information about molecular vibration that could potentially be harnessed as a spectral signature for cellular changes in specific pathologic conditions. In this study we describe a technique using Raman spectroscopy that could potentially be used to diagnose staphylococcal osteomyelitis. Human bone samples were co-cultured with Staphylococcus aureus (S. aureus) and the effects of bacterial growth on bone quality were then monitored using Raman spectroscopy. A major drop in the bone mineral quality and crystallinity was observed in the infected bones compared to the controls. S. aureus infection was also found to alter the collagen cross-linking. Our study shows that specific spectral signatures are present for the cause as well as the effect of staphylococcal osteomyelitis, opening the possibility of developing a useful diagnostic modality for early and rapid diagnosis of this condition.
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影响因子：
4.3
作者：
Judex, Stefan;Boyd, Steve;Rubin, Clinton
通讯作者：
Rubin, Clinton
影响因子：
4.2
作者：
Kim, Grace;Cole, Jacqueline H.;Boskey, Adele L.;Baker, Shefford P.;van der Meulen, Marjolein C. H.
通讯作者：
van der Meulen, Marjolein C. H.
DOI：
10.1001/archpediatrics.2008.545
发表时间：
2009-03-01
影响因子：
--
作者：
Berger, Elizabeth;Saunders, Natasha;Friedman, Jeremy N.
通讯作者：
Friedman, Jeremy N.
影响因子：
4
作者：
Boskey, A
通讯作者：
Boskey, A
骨矿物质成分的改变是糖尿病脚中骨髓炎的早期指示。
影响因子：
16.2
作者：
Esmonde-White KA;Esmonde-White FW;Holmes CM;Morris MD;Roessler BJ
通讯作者：
Roessler BJ