Identification of novel neuroendocrine-specific tumour genes.
Identification of novel neuroendocrine-specific tumour genes.
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DOI：
10.1038/sj.bjc.6604565
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发表时间：
2008-10-21
影响因子：
8.8
通讯作者：
Thommesen, L.
中科院分区：
文献类型：
--
作者：
Hofsli, E.;Wheeler, T. E.;Langaas, M.;Laegreid, A.;Thommesen, L.
神经内分泌肿瘤(Net)包括一组异质性的恶性肿瘤，其病程往往不可预测，治疗选择有限。因此，需要新的诊断、预后和治疗标记物。为了给NET的生物学带来新的曙光，我们通过cDNA转录图谱，试图识别与非NE肿瘤细胞相比，NE上调或下调的基因。一组由6个Net和4个非Net细胞株组成的小组被检查，在被检查的12个 743基因中，我们详细研究了在比较中最显著差异表达的200个基因。除了潜在的新诊断标记物(NEFM、CLDN4、PEROX2)外，结果还指向可能参与肿瘤发生(BEX1、TMEPAI、FOSL1、RAB32)以及与NETs的侵袭、进展和转移过程(MME、STAT3、DCBLD2)有关的基因。实时荧光定量聚合酶链式反应验证结果与芯片结果高度一致。此外，还检测了部分基因的蛋白表达。我们的研究结果为新的研究领域打开了一扇窗，通过发现新的候选基因和蛋白质来进一步研究，以寻找新的预测、预测和治疗标记物。
Neuroendocrine tumours (NETs) comprise a heterogenous group of malignancies with an often unpredictable course, and with limited treatment options. Thus, new diagnostic, prognostic, and therapeutic markers are needed. To shed new lights into the biology of NETs, we have by cDNA transcript profiling, sought to identify genes that are either up- or downregulated in NE as compared with non-NE tumour cells. A panel of six NET and four non-NET cell lines were examined, and out of 12 743 genes examined, we studied in detail the 200 most significantly differentially expressed genes in the comparison. In addition to potential new diagnostic markers (NEFM, CLDN4, PEROX2), the results point to genes that may be involved in the tumorigenesis (BEX1, TMEPAI, FOSL1, RAB32), and in the processes of invasion, progression and metastasis (MME, STAT3, DCBLD2) of NETs. Verification by real time qRT–PCR showed a high degree of consistency to the microarray results. Furthermore, the protein expression of some of the genes were examined. The results of our study has opened a window to new areas of research, by uncovering new candidate genes and proteins to be further investigated in the search for new prognostic, predictive, and therapeutic markers in NETs.
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影响因子：
3.2
作者：
Marcucci, G;Mrózek, K;Bloomfield, CD
通讯作者：
Bloomfield, CD
影响因子：
8
作者：
Koshikawa, K;Osada, H;Takahashi, T
通讯作者：
Takahashi, T
影响因子：
2.3
作者：
Beck, K;Hayashi, K;Boyd, CD
通讯作者：
Boyd, CD
影响因子：
45.3
作者：
Baldus, Claudia D.;Martus, Peter;Hofmann, Wolf K.
通讯作者：
Hofmann, Wolf K.
DOI：
10.1196/annals.1353.056
发表时间：
2006-01-01
期刊：
PHEOCHROMOCYTOMA
影响因子：
--
作者：
Guillemot, Johann;Barbier, Laure;Yon, Laurent
通讯作者：
Yon, Laurent