Morphologic characterization of green fluorescent protein in embryonic, neonatal, and adult transgenic rats.
Morphologic characterization of green fluorescent protein in embryonic, neonatal, and adult transgenic rats.
复制标题
胚胎、新生和成年转基因大鼠绿色荧光蛋白的形态学特征。
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
S. Ookawara
中科院分区：
文献类型：
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作者：
K. Takeuchi;Amornpan Sereemaspun;T. Inagaki;Y. Hakamata;Takashi Kaneko;T. Murakami;Masafumi Takahashi;E. Kobayashi;S. Ookawara
带有报告基因的转基因（Tg）动物是研究细胞谱系和分化为组织的过程的有用模型。我们开发了绿色荧光蛋白（GFP）-Tg 大鼠，它比小鼠更大，更适合移植和干细胞研究。我们发现标记基因表达取决于每个器官和发育阶段。在这项研究中，我们描述了胚胎、新生儿和成年动物的各种组织中的 GFP 表达。脑、肺、肝和胰岛组织中的 GFP 表达仅限于早期发育阶段，但在外分泌胰腺、肾脏、心肌和骨骼肌中持续强表达。据推测诱导普遍存在的蛋白质表达的 CAG 启动子可能是造成表达差异的原因。
Transgenic (Tg) animals with reporter genes are useful models in which to study cell lineage and the process of differentiation into tissues. We developed the green fluorescent protein (GFP)-Tg rat, which is more suitable for transplantation and stem cell research because it is larger than mice. We found that marker gene expression was dependent on each organ and developmental stage. In this study we describe GFP expression in various tissues from embryonic, neonatal, and adult animals. GFP expression in brain, lung, liver, and islet tissues was restricted to early developmental stages, but it was continuously strong in the exocrine pancreas, kidney, and cardiac and skeletal muscles. The CAG promoter that was presumed to induce ubiquitous protein expression might be responsible for the differences in expression.
影响因子：
2.7
作者：
Kisseberth, WC;Brettingen, NT;Sandgren, EP
通讯作者：
Sandgren, EP