MYB and HD-ZIP IV homologs related to trichome formation are involved in epidermal bladder cell development in the halophyte Mesembryanthemum crystallinum L.
MYB and HD-ZIP IV homologs related to trichome formation are involved in epidermal bladder cell development in the halophyte Mesembryanthemum crystallinum L.
复制标题
与毛状体形成相关的 MYB 和 HD-ZIP IV 同源物参与盐生植物 Mesembryanthemum crystallinum L 的表皮膀胱细胞发育。
DOI：
10.1080/1343943x.2017.1279528
复制
发表时间：
2017
影响因子：
2.5
通讯作者：
S.
中科院分区：
文献类型：
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作者：
Roeurn;S.;Hoshino;N. Soejima;K.;Inoue;Y.;Cushman;J.C.;Agarie;S.
常见的冰植物 Mesembryanthemum crystallinum L. 是一种盐生新型功能性叶菜作物，在其气生器官表面发育表皮膀胱细胞 (EBC)。我们之前对野生型和无 EBC 突变体的生理特征的研究表明，EBC 可以螯合盐并维持光合活跃组织内的离子稳态。 EBC 被认为是一种改良的毛状体；然而，控制普通制冰厂 EBC 发育的分子机制尚未完全了解。在这里，我们分析了19个棉纤维相关基因同源物和8个拟南芥发育相关基因的稳态mRNA丰度，发现MYB转录因子同源物（McMYB2）和aGLABRA2样基因（McC4HDZ）在野生型植物中优先表达，而假定的TRIPTYCHON（McTRY）和CAPRICE样基因（McCPC）优先表达在EBC突变体中。确定了这些同源物的全长cDNA序列，并将含有McC4HDZ和McMYB2的构建体引入拟南芥无毛突变体和野生型植物中。野生型拟南芥中 McMYB2 的过度表达会增加毛状体数量，这与毛状体发育相关基因 GLABRA2 (GL2) 的激活有关。此外，McC4HDZ的过表达部分补充了gl2-1无毛突变体中毛状体的发育，并导致野生型拟南芥中毛状体数量增加，这与关键毛状体阳性调节因子GLABRA1（GL1）和透明TESTA GLABRA1（TTG1）的上调有关。这些结果表明McMYB2和McC4HDZ可能在拟南芥毛状体的形成中发挥作用，这意味着普通冰植物和拟南芥毛状体的EBC在其发育过程中可能具有一些共同的分子机制。
The common ice plant,Mesembryanthemum crystallinum L., a halophytic new functional leafy vegetable crop, develops epidermal bladder cells (EBCs) on the surfaces of its aerial organs. Our previous studies of the physiological characteristics of the wild-type and the EBC-less mutant indicated that EBCs sequester salt and maintain ion homeostasis within photosynthetically active tissues. The EBC has been thought to be a modified trichome; however, molecular mechanisms governing EBC development in the common ice plant have not been fully understood. Here, we have analyzed the steady-state mRNA abundance of nineteen cotton fiber-related gene homologs and eightArabidopsistrichome development-related genes, and found that a MYB transcription factor homolog (McMYB2) and aGLABRA2-like gene (McC4HDZ) were preferentially expressed in wild-type plants, whereas a putativeTRIPTYCHON(McTRY)- andCAPRICE-like gene (McCPC) were preferentially expressed in the EBC-mutant. The full-length cDNA sequences of these homologs were determined, and constructs containingMcC4HDZandMcMYB2were introduced into anArabidopsistrichome-less mutant and wild-type plants. Overexpression ofMcMYB2in wild-typeArabidopsisincreased trichome number, associated with activation of the trichome development-related gene,GLABRA2(GL2). Moreover, overexpression ofMcC4HDZpartially complemented trichome development in the trichome-less mutant ofgl2-1, and resulted in increased trichome number in wild-typeArabidopsis, associated with the upregulation of key trichome-positive regulatorsGLABRA1(GL1) andTRANSPARENT TESTA GLABRA1(TTG1). These results suggest thatMcMYB2andMcC4HDZcould be functional inArabidopsistrichome formation, implying that EBCs of the common ice plant and trichomes ofArabidopsismay share some molecular mechanisms in their development.
DOI：
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发表时间：
--
期刊：
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影响因子：
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作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
2.7
作者：
GALWAY, ME;MASUCCI, JD;SCHIEFELBEIN, JW
通讯作者：
SCHIEFELBEIN, JW
影响因子：
4.1
作者：
Cosentino, Cristian;Di Silvestre, Dario;Thiel, Gerhard
通讯作者：
Thiel, Gerhard