Role of G-protein-coupled receptor-related genes in insecticide resistance of the mosquito, Culex quinquefasciatus.
Role of G-protein-coupled receptor-related genes in insecticide resistance of the mosquito, Culex quinquefasciatus.
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DOI：
10.1038/srep06474
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发表时间：
2014-09-29
影响因子：
4.6
通讯作者：
Liu N
中科院分区：
文献类型：
--
作者：
Li T;Liu L;Zhang L;Liu N
G蛋白偶联受体（GPCRs）在昆虫的生理过程中起着重要的调节作用，但其在昆虫抗药性中的具体作用尚不清楚。采用定量RT-PCR和功能基因组学方法，首次研究了致倦库蚊GPCR及其相关基因在蚊虫抗药性中的作用。通过比较115个GPCR相关基因在抗性库蚊和敏感库蚊全基因组水平上的表达，发现1个和3个GPCR相关基因在高抗性库蚊品系HAmCqG 8和MAmCqG 6中表达上调。为了研究这些上调的GPCR相关基因在抗病性中的功能，利用RNAi技术敲低了HAmCqG 8和MAmCqG 6中上调的GPCR相关基因。敲低这4个GPCR相关基因不仅降低了蚊虫对氯菊酯的抗性，而且抑制了4个与杀虫剂抗性相关的P450基因的表达，表明GPCR相关基因在抗性中的作用与抗性P450基因表达的调控有关。这一结果有助于了解分子调控的抗性发展在Cx。致倦肌
G-protein-coupled receptors regulate signal transduction pathways and play diverse and pivotal roles in the physiology of insects, however, the precise function of GPCRs in insecticide resistance remains unclear. Using quantitative RT-PCR and functional genomic methods, we, for the first time, explored the function of GPCRs and GPCR-related genes in insecticide resistance of mosquitoes, Culex quinquefasciatus. A comparison of the expression of 115 GPCR-related genes at a whole genome level between resistant and susceptible Culex mosquitoes identified one and three GPCR-related genes that were up-regulated in highly resistant Culex mosquito strains, HAmCqG8 and MAmCqG6, respectively. To characterize the function of these up-regulated GPCR-related genes in resistance, the up-regulated GPCR-related genes were knockdown in HAmCqG8 and MAmCqG6 using RNAi technique. Knockdown of these four GPCR-related genes not only decreased resistance of the mosquitoes to permethrin but also repressed the expression of four insecticide resistance-related P450 genes, suggesting the role of GPCR-related genes in resistance is involved in the regulation of resistance P450 gene expression. This results help in understanding of molecular regulation of resistance development in Cx. quinquefasciatus.
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影响因子：
11.8
作者：
Asidi A;N'Guessan R;Akogbeto M;Curtis C;Rowland M
通讯作者：
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影响因子：
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DOI：
10.1073/pnas.1003379107
发表时间：
2010-07-06
影响因子：
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作者：
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影响因子：
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