Proteome plasticity in response to persistent environmental change.
Proteome plasticity in response to persistent environmental change.
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DOI：
10.1016/j.molcel.2021.06.028
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发表时间：
2021-08-19
期刊：
影响因子：
16
通讯作者：
Zhou C
中科院分区：
文献类型：
--
作者：
Domnauer M;Zheng F;Li L;Zhang Y;Chang CE;Unruh JR;Conkright-Fincham J;McCroskey S;Florens L;Zhang Y;Seidel C;Fong B;Schilling B;Sharma R;Ramanathan A;Si K;Zhou C
温度是环境的可变组成部分，所有生物都必须处理或适应温度变化。急性温度变化激活细胞应激反应，导致受损蛋白质的重新折叠或移除。然而，生物如何适应长期的温度变化在很大程度上仍未被探索。在这里，我们报道了出芽酵母对长期高温挑战的反应，从伴侣诱导转向温度敏感蛋白的减少，并重新定位其蛋白质组的一部分。令人惊讶的是，我们还发现许多蛋白质采用另一种构象。以Fet3p为例，我们发现温度依赖的构象差异伴随着明显的热稳定性，亚细胞定位，以及重要的细胞功能。我们假设，除了已知的适应机制外，构象可塑性允许一些多肽在环境变化时获得新的生物物理特性和功能。Domnauer等人报道，出芽酵母对长期温度变化的反应是从伴侣诱导转变为耐热性蛋白质的减少和一些蛋白质的重新分配。长期的温度变化也会诱导蛋白质的不同构象，这伴随着一些蛋白质不同的热稳定性、亚细胞定位和细胞功能。
Temperature is a variable component of the environment and all organisms must deal with or adapt to temperature change. Acute temperature change activates cellular stress responses resulting in the refolding or removal of damaged proteins. However, how organisms adapt to long-term temperature change remains largely unexplored. Here, we report that budding yeast responds to long-term high temperature challenge by switching from chaperone induction to the reduction of temperature sensitive proteins and re-localizing a portion of its proteome. Surprisingly, we also find that many proteins adopt an alternative conformation. Using Fet3p as an example, we find that the temperature-dependent conformational difference is accompanied by distinct thermostability, subcellular localization, and importantly, cellular functions. We postulate that in addition to the known mechanisms of adaptation, conformational plasticity allows some polypeptides to acquire new biophysical properties and functions when environmental change endures. Domnauer et al. report that budding yeast responds to long-term temperature change by switching from chaperone induction to the reduction of thermolabile proteins and redistribution of some proteins. Long-term temperature shift also induces alternative protein conformations, which is accompanied by distinct thermostability, subcellular localization, and cellular functions for some proteins.
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影响因子：
2.6
作者：
Funakoshi, Minoru;Hochstrasser, Mark
通讯作者：
Hochstrasser, Mark
影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
影响因子：
4
作者：
Dishman AF;Volkman BF
通讯作者：
Volkman BF
Biopython：用于计算分子生物学和生物信息学的免费 Python 工具。
DOI：
10.1093/bioinformatics/btp163
发表时间：
2009-06-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Cock PJ;Antao T;Chang JT;Chapman BA;Cox CJ;Dalke A;Friedberg I;Hamelryck T;Kauff F;Wilczynski B;de Hoon MJ
通讯作者：
de Hoon MJ
影响因子：
1.2
作者：
Fiala, Gina J.;Schamel, Wolfgang W. A.;Blumenthal, Britta
通讯作者：
Blumenthal, Britta