Integrative genome-wide analysis reveals HLP1, a novel RNA-binding protein, regulates plant flowering by targeting alternative polyadenylation.
Integrative genome-wide analysis reveals HLP1, a novel RNA-binding protein, regulates plant flowering by targeting alternative polyadenylation.
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全基因组综合分析揭示 HLP1（一种新型 RNA 结合蛋白）通过靶向选择性多聚腺苷酸化来调节植物开花
作者：
选择性多腺苷酸化 (APA) 是一种广泛的基因调控机制，与开花有关，但在营养生长到生殖生长转变期间控制特定多聚腺苷酸位点选择的分子基础仍不清楚。在这里，我们将 HLP1（一种 hnRNP A/B 蛋白）描述为拟南芥中前 mRNA 3' 端加工的新型调节因子。遗传分析表明，HLP1 抑制开花基因座 C (FLC)，这是拟南芥开花的关键抑制因子。 HLP1-RNA 相互作用的全基因组图谱表明，HLP1 优先与切割和聚腺苷酸化位点周围富含 A 和 U 的元件结合，暗示其在 3' 末端形成中的作用。我们发现 HLP1 在参与 RNA 代谢和开花的转录本中显着富集。对 Poly (A) 位点使用情况的全面分析表明，HLP1 突变导致数千个 Poly (A) 位点移位。开花调节因子 FCA（HLP1 的直接靶标）中从远端到近端的 Poly (A) 位点移位会导致 FLC 上调并延迟开花。我们的结果阐明 HLP1 是参与 3' 末端加工的新因子，并通过靶向 APA 控制生殖时间。
Alternative polyadenylation (APA) is a widespread mechanism for gene regulation and has been implicated in flowering, but the molecular basis governing the choice of a specific poly (A) site during the vegetative-to-reproductive growth transition remains unclear. Here we characterize HLP1, an hnRNP A/B protein as a novel regulator for pre-mRNA 3′-end processing in Arabidopsis. Genetic analysis reveals that HLP1 suppresses Flowering Locus C (FLC), a key repressor of flowering in Arabidopsis. Genome-wide mapping of HLP1-RNA interactions indicates that HLP1 binds preferentially to A-rich and U-rich elements around cleavage and polyadenylation sites, implicating its role in 3′-end formation. We show HLP1 is significantly enriched at transcripts involved in RNA metabolism and flowering. Comprehensive profiling of the poly (A) site usage reveals that HLP1 mutations cause thousands of poly (A) site shifts. A distal-to-proximal poly (A) site shift in the flowering regulator FCA, a direct target of HLP1, leads to upregulation of FLC and delayed flowering. Our results elucidate that HLP1 is a novel factor involved in 3′-end processing and controls reproductive timing via targeting APA.
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