Targeted re-sequencing confirms the importance of chemosensory genes in aphid host race differentiation.
Targeted re-sequencing confirms the importance of chemosensory genes in aphid host race differentiation.
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DOI：
10.1111/mec.13818
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发表时间：
2017-01
影响因子：
4.9
通讯作者：
Butlin RK
中科院分区：
文献类型：
--
作者：
Eyres I;Duvaux L;Gharbi K;Tucker R;Hopkins D;Simon JC;Ferrari J;Smadja CM;Butlin RK
植食性昆虫的宿主相关种族提供了一个模型，用于理解适应新环境如何导致生殖隔离和物种形成，最终使我们能够将基因流动的障碍与适应性差异的原因联系起来。豌豆蚜（Acyrthosiphon pisum）包括专门研究豆科植物的寄主品种，并提供了一个独特的系统，用于研究多样化的早期阶段，沿着遗传和相关的适应性分歧的梯度。由于宿主选择产生选择性交配，理解选择的潜在机制将直接有助于理解物种形成。由于豌豆蚜虫的宿主选择可能是由嗅觉和味觉介导的，我们使用捕获测序和SNP基因分型来测试化学感受基因在8种宿主植物物种之间的分化中的作用，并在西欧的多个地点取样。我们显示了高分化的化学感受位点相对于控制位点在一组广泛的豌豆蚜虫种族和地方，使用基于主成分分析的无模型方法。嗅觉和味觉受体形成了大多数高度分化的基因，包括在以前的研究中已经被确定为离群值的基因座，该研究集中在三个最密切相关的宿主种族上。一致的迹象表明，化学感受基因可能是很好的候选人，当地的适应和障碍，在豌豆蚜虫的基因流开辟了道路，以进一步调查，旨在了解其对基因流的影响，并确定其精确的功能，响应宿主植物代谢产物。
Host‐associated races of phytophagous insects provide a model for understanding how adaptation to a new environment can lead to reproductive isolation and speciation, ultimately enabling us to connect barriers to gene flow to adaptive causes of divergence. The pea aphid (Acyrthosiphon pisum) comprises host races specializing on legume species and provides a unique system for examining the early stages of diversification along a gradient of genetic and associated adaptive divergence. As host choice produces assortative mating, understanding the underlying mechanisms of choice will contribute directly to understanding of speciation. As host choice in the pea aphid is likely mediated by smell and taste, we use capture sequencing and SNP genotyping to test for the role of chemosensory genes in the divergence between eight host plant species across the continuum of differentiation and sampled at multiple locations across western Europe. We show high differentiation of chemosensory loci relative to control loci in a broad set of pea aphid races and localities, using a model‐free approach based on principal component analysis. Olfactory and gustatory receptors form the majority of highly differentiated genes and include loci that were already identified as outliers in a previous study focusing on the three most closely related host races. Consistent indications that chemosensory genes may be good candidates for local adaptation and barriers to gene flow in the pea aphid open the way to further investigations aiming to understand their impact on gene flow and to determine their precise functions in response to host plant metabolites.
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影响因子：
10.7
作者：
Duvaux L;Geissmann Q;Gharbi K;Zhou JJ;Ferrari J;Smadja CM;Butlin RK
通讯作者：
Butlin RK
影响因子：
56.9
作者：
Barrett, Rowan D. H.;Rogers, Sean M.;Schluter, Dolph
通讯作者：
Schluter, Dolph
影响因子：
3.8
作者：
Caillaud, M. C.;Via, S.
通讯作者：
Via, S.
影响因子：
3.3
作者：
Foll, Matthieu;Gaggiotti, Oscar
通讯作者：
Gaggiotti, Oscar
影响因子：
7.7
作者：
Excoffier, Laurent;Lischer, Heidi E. L.
通讯作者：
Lischer, Heidi E. L.