A genome-wide association study on androstenone levels in pigs reveals a cluster of candidate genes on chromosome 6.
A genome-wide association study on androstenone levels in pigs reveals a cluster of candidate genes on chromosome 6.
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DOI：
10.1186/1471-2156-11-42
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发表时间：
2010-05-20
期刊：
影响因子：
2.9
通讯作者：
Harlizius B
中科院分区：
文献类型：
--
作者：
Duijvesteijn N;Knol EF;Merks JW;Crooijmans RP;Groenen MA;Bovenhuis H;Harlizius B
在许多国家，雄性小猪在出生后不久就被阉割，因为一部分未阉割的雄性小猪产生的肉具有令人不快的味道和气味。公猪粪臭的主要成分是雄烯酮和粪臭素。这项高密度全基因组关联研究的目的是确定与雄甾烯酮水平相关的单核苷酸多态性（SNP）在商业父系猪。鉴定导致公猪污点的主要遗传效应将通过育种加速公猪污点的减少，最终消除阉割的需要。Illumina Porcine 60 K +SNP Beadchip对987头来自商业杜洛克父系的雄甾烯酮浓度不同的猪进行基因分型。对47,897个SNP的关联分析显示，猪染色体SSC 1和SSC 6上的37个SNP显著影响脂肪组织中的雄甾烯酮水平。其中，5个最显著的SNPs共解释了雄烯酮遗传变异的13.7%。在SSC 6上，显示10 Mb的较大区域与雄甾烯酮相关，覆盖了可能参与雄激素合成和代谢的几个候选基因。除了已知的候选基因，如细胞色素P450 A19（CYP 2A 19）、磺基转移酶SULT 2A 1和SULT 2B 1之外，还有细胞色素P450 CYP 2基因亚家族和羟基类固醇脱氢酶的新成员（HSD 17 B14）。此外，编码促黄体激素β链的基因其在青春期开始时诱导睾丸间质细胞中类固醇合成，映射到SSC 6上的该区域。有趣的是，编码LH α链的基因也位于SSC 1上的一个高度显著区域。这项研究揭示了高分辨率的基因组的几个区域负责在完整的公猪雄烯酮水平的变化。在该猪商业育种系中，确定了对雄甾烯酮浓度产生中度至较大影响的SSC 1和SSC 6的主要遗传因素。已知和新的候选基因尤其聚集在SSC 6上。对于最显著的SNP变体之一，两种纯合基因型之间超过消费者接受阈值的动物比例的差异高达15.6%。
In many countries, male piglets are castrated shortly after birth because a proportion of un-castrated male pigs produce meat with an unpleasant flavour and odour. Main compounds of boar taint are androstenone and skatole. The aim of this high-density genome-wide association study was to identify single nucleotide polymorphisms (SNPs) associated with androstenone levels in a commercial sire line of pigs. The identification of major genetic effects causing boar taint would accelerate the reduction of boar taint through breeding to finally eliminate the need for castration. The Illumina Porcine 60K+SNP Beadchip was genotyped on 987 pigs divergent for androstenone concentration from a commercial Duroc-based sire line. The association analysis with 47,897 SNPs revealed that androstenone levels in fat tissue were significantly affected by 37 SNPs on pig chromosomes SSC1 and SSC6. Among them, the 5 most significant SNPs explained together 13.7% of the genetic variance in androstenone. On SSC6, a larger region of 10 Mb was shown to be associated with androstenone covering several candidate genes potentially involved in the synthesis and metabolism of androgens. Besides known candidate genes, such as cytochrome P450 A19 (CYP2A19), sulfotransferases SULT2A1, and SULT2B1, also new members of the cytochrome P450 CYP2 gene subfamilies and of the hydroxysteroid-dehydrogenases (HSD17B14) were found. In addition, the gene encoding the ß-chain of the luteinizing hormone (LHB) which induces steroid synthesis in the Leydig cells of the testis at onset of puberty maps to this area on SSC6. Interestingly, the gene encoding the α-chain of LH is also located in one of the highly significant areas on SSC1. This study reveals several areas of the genome at high resolution responsible for variation of androstenone levels in intact boars. Major genetic factors on SSC1 and SSC6 showing moderate to large effects on androstenone concentration were identified in this commercial breeding line of pigs. Known and new candidate genes cluster especially on SSC6. For one of the most significant SNP variants, the difference in the proportion of animals surpassing the threshold of consumer acceptance between the two homozygous genotypes was as much as 15.6%.
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影响因子：
120.7
作者：
Pearson, Thomas A.;Manolio, Teri A.
通讯作者：
Manolio, Teri A.
影响因子：
7.1
作者：
Candek-Potokar, M;Zlender, B;Bonneau, M
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影响因子：
1.8
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2.4
作者：
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56.9
作者：
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通讯作者：
Altshuler, D