Estrogen-Specific Sulfotransferase (SULT1E1) in Bovine Placentomes: Inverse Levels of mRNA and Protein in Uninucleated Trophoblast Cells and Trophoblast Giant Cells1
Estrogen-Specific Sulfotransferase (SULT1E1) in Bovine Placentomes: Inverse Levels of mRNA and Protein in Uninucleated Trophoblast Cells and Trophoblast Giant Cells1
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牛胎盘素中的雌激素特异性磺基转移酶 (SULT1E1)：无核滋养层细胞和滋养层巨细胞中 mRNA 和蛋白质的反向水平1
DOI：
10.1095/biolreprod.114.118760
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Fürbass R
中科院分区：
文献类型：
--
作者：
Polei M;Viergutz T;Tomek W;Schuler G ;Fürbass R
牛的滋养层产生大量的雌激素。在母体和胎儿血液中，雌激素主要以磺化形式存在，其不能与雌激素受体（ESRs）结合。然而，雌激素可能作为局部因素在ESR阳性滋养层细胞或在相邻的caruncular上皮，除了ESR高度表达类固醇硫酸酯酶。雌激素磺化是由胞质酶SULT 1 E1催化的。以前的研究清楚地表明滋养层是雌激素磺化的主要部位。然而，对SULT 1 E1细胞定位的研究产生了相互矛盾的结果。原位杂交研究检测到SULT 1 E1 mRNA仅在滋养层巨细胞（TGC），而在免疫组化实验中，SULT 1 E1蛋白几乎仅限于单核滋养层细胞（UTCs）。这项工作的目的是通过分析SULT 1 E1在分离的UTCs和TGC中的表达来解决这一冲突。使用优化的荧光激活细胞分选程序从四个牛胎盘（妊娠第118-130天）获得UTCs和TGC的高度富集池。通过定量RT-PCR和Western印迹实验分析UTC和TGC池，以分别测量SULT 1 E1转录物和蛋白质的量。与先前发表的结果相反，SULT 1 E1转录本和SULT 1 E1蛋白都清楚地存在于UTC和TGC库中。然而，一些证据表明，较高的转录本浓度在TGCs和较高量的蛋白质在UTCs。因此，我们的研究结果解决了早期研究中SULT 1 E1定位的相互矛盾的结果，并表明转录后机制在TGC分化过程中SULT 1 E1表达的控制中起着重要作用。
The bovine trophoblast produces significant amounts of estrogens. In maternal and fetal blood, estrogens occur predominantly in sulfonated forms, which are unable to bind to estrogen receptors (ESRs). However, estrogens may act as local factors in ESR-positive trophoblast cells or in the adjacent caruncular epithelium, which in addition to ESR highly expresses steroid sulfatase. Estrogen sulfonation is catalyzed by the cytosolic enzyme SULT1E1. Previous studies clearly indicated the trophoblast as the primary site of estrogen sulfonation. However, investigations into the cellular localization of SULT1E1 yielded conflicting results. In situ hybridization studies detectedSULT1E1mRNA only in trophoblast giant cells (TGCs), whereas in immunohistochemical experiments the SULT1E1 protein was virtually restricted to uninucleated trophoblast cells (UTCs). The aim of this work was to resolve this conflict by analyzing SULT1E1 expression in isolated UTCs and TGCs. Highly enriched pools of UTCs and TGCs were obtained from four bovine placentas (Days 118–130 of gestation) using an optimized fluorescence-activated cell sorting procedure. UTC and TGC pools were analyzed by quantitative RT-PCR and Western blot experiments to measure the amounts of SULT1E1 transcript and protein, respectively. In contrast to previously published results, bothSULT1E1transcript and SULT1E1 protein were clearly present in the UTC and TGC pools. However, some evidence indicated a higher transcript concentration in TGCs and a higher amount of protein in UTCs. Thus, our results resolve the conflicting results on the localization of SULT1E1 from earlier studies and suggest that posttranscriptional mechanisms play an important role in the control of SULT1E1 expression during TGC differentiation.
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近期母牛和胎儿血浆中的游离类固醇和结合类固醇。
影响因子：
3.6
作者：
B. Hoffmann;W. C. Wagner;T. Gimenez
通讯作者：
T. Gimenez
雌激素受体β (ERbeta) 在牛胎盘中的表达与 ERα 不同。
影响因子：
1.8
作者：
G. Schuler;U. Teichmann;A. Taubert;K. Failing;B. Hoffmann
通讯作者：
B. Hoffmann
影响因子：
2.2
作者：
Schlafer, DH;Fisher, PJ;Davies, CJ
通讯作者：
Davies, CJ
影响因子：
3.8
作者：
Khatri, P.;Frenette, G.;Schuler, G.
通讯作者：
Schuler, G.
牛胎盘成熟机制的形态和功能方面。
DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
J. Woicke;H. Schoon;W. Heuwieser;L.;E. Grunert
通讯作者：
E. Grunert