Elucidation of gastrointestinal dysfunction in response to irradiation using metabolomics
Elucidation of gastrointestinal dysfunction in response to irradiation using metabolomics
复制标题
使用代谢组学阐明放射反应引起的胃肠功能障碍
DOI：
10.1016/j.bbrep.2020.100789
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发表时间：
2020
影响因子：
2.7
通讯作者：
Sasaki Ryohei
中科院分区：
文献类型：
--
作者：
Salah Mohammed;Osuga Saki;Nakahana Makiko;Irino Yasuhiro;Shinohara Masakazu;Shimizu Yasuyuki;Mukumoto Naritoshi;Akasaka Hiroaki;Nakaoka Ai;Miyawaki Daisuke;Ishihara Takeaki;Yoshida Kenji;Okamoto Yoshiaki;Sasaki Ryohei
胃肠道毒性通常继发于意外或治疗性辐射暴露。然而，腹部辐射后肠道代谢物的变化仍然不明确。在本研究中，C57BL/6小鼠分别接受0、2和20Gy射线照射。在X射线照射前，每只小鼠的头部和胸部都用铅盾盖住。照射后24小时处死小鼠，取肠道组织，用气相色谱-质谱仪(GC-MS)测定代谢物。我们对肠道组织中代谢物的综合分析在照射后检测到44种代谢物，包括氨基酸、碳水化合物、有机酸和糖。肠道组织中的氨基酸水平随着辐射剂量的增加而逐渐上升，这可能是氧化应激的一个指标。我们的发现提出，氨基酸代谢可能是开发治疗方法的潜在靶点，以减轻或减轻辐射暴露引起的氧化应激相关胃肠毒性的有害影响。
Gastrointestinal toxicity is frequently observed secondary to accidental or therapeutic radiation exposure. However, the variation in the intestinal metabolites after abdominal radiation exposure remains ambiguous. In the present study, C57BL/6 mice were exposed to 0, 2, and 20 Gy irradiation dose. The Head and chest of each mouse were covered with a lead shield before x-ray irradiation. 24 h post-irradiation treatment, intestinal tissue of each mouse was excised and prepared for metabolites measurement using gas chromatography-mass spectrometry (GC-MS). Our comprehensive analysis of metabolites in the intestinal tissues detected 44 metabolites after irradiation, including amino acids, carbohydrates, organic acids, and sugars. Amino acid levels in the intestinal tissue gradually rose, dependent on the radiation dose, perhaps as an indication of oxidative stress. Our findings raise the possibility that amino acid metabolism may be a potential target for the development of treatments to alleviate or mitigate the harmful effects of oxidative stress-related gastrointestinal toxicity due to radiation exposure.
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影响因子：
--
作者：
Ghosh SP;Singh R;Chakraborty K;Kulkarni S;Uppal A;Luo Y;Kaur P;Pathak R;Kumar KS;Hauer-Jensen M;Cheema AK
通讯作者：
Cheema AK
影响因子：
5.5
作者：
Howard, Alison;Tahir, Imran;Hirst, Barry H.
通讯作者：
Hirst, Barry H.
肠道菌群衍生的代谢组血浆标志物，用于先前的辐射损伤。
DOI：
10.1016/j.ijrobp.2014.10.023
发表时间：
2015-02-01
影响因子：
7
作者：
Broin, Pilib O.;Vaitheesvaran, Bhavapriya;Saha, Subhrajit;Hartil, Kirsten;Chen, Emily I.;Goldman, Devorah;Fleming, William Harv;Kurland, Irwin J.;Guha, Chandan;Golden, Aaron
通讯作者：
Golden, Aaron
DOI：
10.3390/ph11010002
发表时间：
2017-12-23
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
Ikwegbue PC;Masamba P;Oyinloye BE;Kappo AP
通讯作者：
Kappo AP
影响因子：
7.4
作者：
Patterson, Andrew D.;Li, Henghong;Idle, Jeffrey R.
通讯作者：
Idle, Jeffrey R.