Improved accuracy in the determination of field-flow fractionation elution volumes.
Improved accuracy in the determination of field-flow fractionation elution volumes.
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提高了场流分级洗脱体积测定的准确性。
DOI：
10.1016/s0021-9673(01)87187-9
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Caldwell,KD
中科院分区：
文献类型：
--
作者：
Li,JM;Caldwell,KD
场流动分馏(FFF)系统的常规操作包括在恒定的载体流量下进行分析；在进样后流动被暂时中断，以便使其在外加磁场下保持平衡。保留率分别计算为未保留物种和感兴趣样品的洗脱时间之比。这种基于时间的保留只有在运行过程中所有时间都精确知道流量的情况下才有效。经常与FFF设备一起使用的蠕动泵的输出量在时间上发生了不可预测的变化。此外，在松弛后启动流动被证明在系统调整到操作压力时会导致显著的瞬变行为周期。通过将滞留测量建立在流出物重量而不是时间上，可以消除这些和其他流量变化作为误差来源。为此，电子天平与系统的计算机连接，以便在FFF分析过程中连续监测探测器响应/流出物重量数据对。
Conventional operation of field-flow fractionation (FFF) systems involves carrying out the analysis at a constant flow of carrier; the flow is temporarily interrupted after injection of a sample in order to permit its equilibration under the applied field. Retention is calculated as the ratio of elution times for a non-retained species and the sample of interest, respectively. Such time-based retentions are only valid if the flow-rate is precisely known at all times during the run. The peristaltic pumps often used with FFF equipment are shown to have an output which varies unpredictably in time. Furthermore, initiation of flow after relaxation is shown to result in significant periods of transient behaviour while the system adjusts to the operating pressure. These and other variations in flow-rate can be eliminated as sources of error by basing the retention measurement on effluent weight, rather than on time. For this purpose, an electronic balance is interfaced with the system's computer, so that detector response/effluent weight data pairs are continuously monitored during the course of the FFF analysis.
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DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
M. Schure
通讯作者：
M. Schure
影响因子：
7.4
作者：
K. Caldwell;M. N. Myers
通讯作者：
M. N. Myers
影响因子：
7.4
作者：
J. Giddings;K. Caldwell;J. F. Moellmer;T. H. Dickinson;M. N. Myers;Michel Martin
通讯作者：
Michel Martin
流场流分馏中溶质弛豫导致的峰移和畸变
影响因子：
7.4
作者：
Frank J. Yang;M. N. Myers;J. Giddings
通讯作者：
J. Giddings
热场流分级中的非平衡和多分散性峰展宽
DOI：
10.1080/01483917908060055
发表时间：
1979
影响因子：
1.3
作者：
Michel Martin;M. N. Myers;J. Giddings
通讯作者：
J. Giddings