Astrocytes Express N-Methyl-D-Aspartate Receptor Subunits in Development, Ischemia and Post-Ischemia
Astrocytes Express N-Methyl-D-Aspartate Receptor Subunits in Development, Ischemia and Post-Ischemia
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星形胶质细胞在发育、缺血和缺血后表达 N-甲基-D-天冬氨酸受体亚基
DOI：
10.1007/s11064-010-0325-x
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发表时间：
2010-11
影响因子：
4.4
通讯作者：
Chen, Jianguo
中科院分区：
文献类型：
--
作者：
Yu, Albert Cheung-Hoi;Li, Hui Li;Zhao, Rui;Yang, Li Tao;Dong, Yan;Yue, Xin;Ma, Yao Ying;Wang, Zhuo;Chen, Jianguo
N-甲基-D-天冬氨酸受体（NMDA-R）在星形胶质细胞中的表达存在争议。该受体通常被认为是神经元特异性的。我们发现，原代培养的星形胶质细胞在发育、缺血和缺血后差异表达NMDA-R亚基NR 1、NR 2A和NR 2B的mRNA。一周大的培养物表达可检测到的NR 1 mRNA，在2周时显著下降，在4周时几乎检测不到。NR 2A和NR 2B mRNA在1 ~ 2周时均显著上调。4周时，缺血2 h引起NR 1和NR 2B mRNA表达显著上调，缺血6 h引起NR 2A mRNA表达下调。缺血后3 h，仅NR 1 mRNA表达增加。缺血诱导的主要NMDA-R效应，一氧化氮合酶1的表达，这是不受AMPA-R拮抗剂CNQX，但剂量依赖性抑制NMDA-R特异性拮抗剂MK-801。这些结果反映了星形胶质细胞在无神经元存在的情况下也能表达诱导型功能性NMDA受体。
The expression of the N-methyl-D-aspartate receptor (NMDA-R) in astrocytes is controversial. The receptor is commonly considered neuron-specific. We showed that astrocytes in primary cultures differentially expressed mRNA of NMDA-R subunits, NR1, NR2A and NR2B, in development, ischemia and post-ischemia. One-week-old cultures expressed detectable NR1 mRNA, which fell significantly at 2 weeks and became barely detectable at 4 weeks. NR2A and NR2B mRNA were both significantly up-regulated from 1 to 2 weeks. In 4 weeks, 2 h of ischemia caused a significant up-regulation of NR1 and NR2B mRNA; while 6 h caused down-regulation of NR2A mRNA. Under 3 h of post-ischemia, only NR1 mRNA was increased. Ischemia induced the expression of major NMDA-R effecter, nitric oxide synthase 1, which was unaffected by AMPA-R antagonist CNQX, but dose-dependently inhibited by NMDA-R specific antagonist MK-801. These findings reflected that astrocyte could express inducible functional NMDA receptors without the presence of neurons.
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影响因子：
4.7
作者：
A. Yu;E. Hertz;L. Hertz
通讯作者：
A. Yu;E. Hertz;L. Hertz
影响因子：
2.9
作者：
J. Morán;A. J. Patel
通讯作者：
J. Morán;A. J. Patel
影响因子：
2.5
作者：
Small, DL;Poulter, MO;Morley, P
通讯作者：
Morley, P
影响因子：
4.4
作者：
Zhi Li;Qiang-Bing Li;Chun Xiao Sun;L. Hertz;A. Yu
通讯作者：
Zhi Li;Qiang-Bing Li;Chun Xiao Sun;L. Hertz;A. Yu
DOI：
10.1073/pnas.86.13.5159
发表时间：
1989-07-01
影响因子：
11.1
作者：
KNOWLES, RG;PALACIOS, M;MONCADA, S
通讯作者：
MONCADA, S