Complete genome sequence of a tobacco isolate of the tobacco vein banding mosaic virus strain prevailing in China
Complete genome sequence of a tobacco isolate of the tobacco vein banding mosaic virus strain prevailing in China
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中国流行的烟草脉带花叶病毒株烟草分离株的全基因组序列
DOI：
10.1007/s00705-009-0570-y
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发表时间：
2010-02
影响因子：
2.7
通讯作者：
Zhu, X-P
中科院分区：
文献类型：
--
作者：
Wang, H-Y;Zhu, T-S;Cui, T-T;Hou, S-S;Yin, X.;Li, Xiang-Dong;Lei, L-P;Zhu, X-P
烟草脉带花叶病毒（Tobacco vein banding mosaic virus，TVBMV）是马铃薯Y病毒属（Potyvirus）中最大的一种植物病毒.在我国烟区发病率呈上升趋势。TVBMV分离物可分为3个遗传群，与其地理来源相对应。我们报道了TVBMV分离物YND的全基因组序列，该分离物具有独特的NIb/CP切割位点。本研究对TVBMV中国流行株系河南烟草分离物HN 39的全基因组序列进行了测定和分析。HN 39的寄主范围与YND相似，但在烟草上引起轻微的脉带症状。萨姆森TVBMV-HN 39的基因组由9，570个核苷酸组成，不包括poly（A）尾。它含有一个9，240个核苷酸的开放阅读框，编码一个3，079个氨基酸的多聚蛋白。NIb/CP的推定NIa-Pro切割位点是Q/G。HN 39株与YND株全基因组核苷酸序列同源性为90.0%，氨基酸序列同源性为95.4%。对于其他马铃薯Y病毒组，HN 39与野生番茄花叶病毒（WTMV）在全基因组水平上具有最高的同一性，而不同的基因与不同的马铃薯Y病毒组具有最高的同一性。这是TVBMV的第二个全基因组序列。
Tobacco vein banding mosaic virus(TVBMV) is a species of the largest plant virus genusPotyvirus. Its incidence has been increasing in Chinese tobacco-growing area. TVBMV isolates can be clustered into three genetic groups that are corresponding with their geographical origin. We have reported the complete genomic sequence of TVBMV isolate YND with unique NIb/CP cleavage site. Here, we determined and analyzed the complete genomic sequence of isolate HN39, which was collected from tobacco in Henan Province and represented Chinese prevalent strain of TVBMV. HN39 has similar host range with YND, but induce mild vein banding symptom inNicotiana tabacumcv. Samsun. The genome of TVBMV-HN39 is composed of 9,570 nucleotides, excluding the poly(A) tail. It contains a large ORF of 9,240 nucleotides and encode a polyprotein of 3,079 amino acids. The putative NIa-Pro cleavage site for NIb/CP is Q/G. The identities between the complete genomes of isolates HN39 and YND were 90.0% at nucleotide level and 95.4% at amino acid level. As for other potyviruses, HN39 shared the highest identity with wild tomato mosaic virus (WTMV) at complete genomic level, while different genes shared the highest identities with different potyviruses. This is the second complete genomic sequence of TVBMV reported.
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DOI：
--
发表时间：
1966-02
期刊：
--
影响因子：
--
作者：
W. Chin
通讯作者：
W. Chin
影响因子：
4.5
作者：
B. B. Reddick-B.;M. H. Collins-Shepard;R. Christie;G. Gooding
通讯作者：
B. B. Reddick-B.;M. H. Collins-Shepard;R. Christie;G. Gooding
影响因子：
9.5
作者：
Kumar, S;Tamura, K;Nei, M
通讯作者：
Nei, M
影响因子：
2.7
作者：
Y.-P. Tian;J.-L. Liu;X. Yu;L. Lei;X.-P. Zhu;J. Valkonen;X. Li
通讯作者：
Y.-P. Tian;J.-L. Liu;X. Yu;L. Lei;X.-P. Zhu;J. Valkonen;X. Li
影响因子：
2.7
作者：
Z. Fan;H. Chen;S. Cai;C. Deng;W. Wang;X. Liang;H. Li
通讯作者：
Z. Fan;H. Chen;S. Cai;C. Deng;W. Wang;X. Liang;H. Li