MicroRNA expression as a predictive marker for gemcitabine response after surgical resection of pancreatic cancer.
MicroRNA expression as a predictive marker for gemcitabine response after surgical resection of pancreatic cancer.
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胰腺癌手术切除后，microRNA表达是吉西他滨反应的预测标记。
DOI：
10.1245/s10434-011-1602-x
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发表时间：
2011-08
影响因子：
3.7
通讯作者：
Tanaka M
中科院分区：
文献类型：
--
作者：
Ohuchida K;Mizumoto K;Kayashima T;Fujita H;Moriyama T;Ohtsuka T;Ueda J;Nagai E;Hashizume M;Tanaka M
为了改善胰腺癌切除后患者的预后，应为特定的患者亚群提供最合适、最有效的治疗。我们的目标是确定有前景的 microRNA 作为标记物来预测胰腺癌切除患者对吉西他滨的反应。建立了两种吉西他滨耐药胰腺癌细胞系，并通过定量逆转录聚合酶链反应（qRT-PCR）进行整体 microRNA 表达分析。选择 11 种 miRNA 作为假定的预测标记，并通过从 90 名胰腺癌切除后接受或未接受吉西他滨治疗的患者获得的福尔马林固定、石蜡包埋的宏观解剖样本进行分析。我们鉴定了 24 种 microRNA，其表达在吉西他滨耐药细胞中发生了改变。 qRT-PCR 分析显示，吉西他滨治疗组中 miR-142-5p 和 miR-204 高表达的患者比 miR-142-5p (P = 0.0077) 和 miR-204 (P = 0.0054) 表达低的患者的生存时间显着更长。这在未治疗组中未见。多变量分析显示，miR-142-5p 表达仅在接受吉西他滨治疗的患者中是独立的预后标志物（P = 0.034）。 miR-142-5p 是胰腺癌切除患者吉西他滨反应的一个有前途的预测标志物。
To improve the prognosis of patients after resection of pancreatic cancer, the most appropriate and efficient treatment should be provided to specific subsets of patients. Our aim was to identify promising microRNAs as markers to predict responses to gemcitabine in patients with resected pancreatic cancer. Two gemcitabine-resistant pancreatic cancer cell lines were established, and global microRNA expression analyses was performed by quantitative reverse transcription–polymerase chain reaction (qRT-PCR). Eleven miRNAs were selected as putative predictive markers and analyzed by means of macrodissected formalin-fixed, paraffin-embedded samples obtained from 90 patients with or without gemcitabine treatment after resection of pancreatic cancer. We identified 24 microRNAs whose expression was altered in gemcitabine-resistant cells. qRT-PCR analyses showed that patients with high miR-142-5p and miR-204 expression had significantly longer survival times than those with low miR-142-5p (P = 0.0077) and miR-204 (P = 0.0054) expression in the gemcitabine-treated group. This was not seen in the nontreated group. Multivariate analyses showed that miR-142-5p expression was an independent prognostic marker only in patients treated with gemcitabine (P = 0.034). miR-142-5p is a promising predictive marker for gemcitabine response in patients with resected pancreatic cancer.
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影响因子：
45.3
作者：
Van Cutsem, Eric;Vervenne, Walter L.;Moore, Malcolm J.
通讯作者：
Moore, Malcolm J.
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作者：
Hoffmann, Andreas-Claudius;Mori, Ryutaro;Danenberg, Peter V.
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Lagos-Quintana, M;Rauhut, R;Tuschl, T
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影响因子：
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作者：
Chen, Lei;Yan, He-Xin;Wang, Hong-Yang
通讯作者：
Wang, Hong-Yang
影响因子：
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作者：
Moore, Malcolm J.;Goldstein, David;Parulekar, Wendy
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