In Vivo Single-Molecule Detection of Nanoparticles for Multiphoton Fluorescence Correlation Spectroscopy to Quantify Cerebral Blood Flow.
In Vivo Single-Molecule Detection of Nanoparticles for Multiphoton Fluorescence Correlation Spectroscopy to Quantify Cerebral Blood Flow.
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DOI：
10.1021/acs.nanolett.0c02280
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发表时间：
2020-08-12
期刊：
影响因子：
10.8
通讯作者：
Richards CI
中科院分区：
文献类型：
--
作者：
Fu X;Sompol P;Brandon JA;Norris CM;Wilkop T;Johnson LA;Richards CI
我们提出了荧光纳米颗粒的多光子体内荧光相关光谱（FCS）的应用，以优异的空间和时间分辨率测量脑血流。通过检测活体小鼠复杂血管结构内的单个纳米颗粒，这种新方法能够量化脉管系统内发生的纳米颗粒动力学，同时测量大脑中的血流特性。除了提供高分辨率血流测量之外，该方法还可以实时量化纳米颗粒浓度、降解和运输。该方法能够以亚微米分辨率量化每个像素的流速，从而能够监测流速随动物生理状况变化的动态变化。使用 FCS 扫描激发光束可提供小鼠大脑 300 μm 深处毛细血管的逐像素流速图和亚血管分辨率。
We present the application of multiphoton in vivo fluorescence correlation spectroscopy (FCS) of fluorescent nanoparticles for the measurement of cerebral blood flow with excellent spatial and temporal resolution. Through the detection of single nanoparticles within the complex vessel architecture of a live mouse, this new approach enables the quantification of nanoparticle dynamics occurring within the vasculature along with simultaneous measurements of blood flow properties in the brain. In addition to providing high resolution blood flow measurements, this approach enables real-time quantification of nanoparticle concentration, degradation, and transport. This method is capable of quantifying flow rates at each pixel with submicron resolution to enable monitoring of dynamic changes in flow rates in response to changes in the animal’s physiological condition. Scanning the excitation beam using FCS provides pixel by pixel mapping of flow rates with subvessel resolution across capillaries 300 μm deep in the brains of mice.
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