Time- and Dose-Dependent Effects of Pulsed Ultrasound on Dermal Repair in Diabetic Mice.
Time- and Dose-Dependent Effects of Pulsed Ultrasound on Dermal Repair in Diabetic Mice.
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DOI：
10.1016/j.ultrasmedbio.2020.12.024
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发表时间：
2021-04
影响因子：
2.9
通讯作者：
Hocking DC
中科院分区：
文献类型：
--
作者：
Vander Horst MA;Raeman CH;Dalecki D;Hocking DC
慢性伤口，包括糖尿病性溃疡、腿部溃疡和压力性溃疡，在世界范围内造成了重大的医疗保健负担。一些证据表明，超声可以促进软组织修复。然而，个体之间的治疗反应不同，从而限制了临床转化。在这里，使用慢性糖尿病伤口的小鼠模型评估脉冲超声对皮肤伤口愈合的影响。开发了一种超声暴露系统，用于对遗传性糖尿病小鼠的全层切除伤口进行日常超声暴露。将伤口暴露于1 MHz超声（2 ms脉冲，100 Hz PRF，0-0.4 MPa）2或3周。肉芽组织厚度和伤口再上皮化随着超声压力振幅的增加而增加。损伤后两周，肉芽组织厚度和上皮向内生长的显着增加，观察到响应于1 MHz的脉冲超声在0.4 MPa。暴露于0.4 MPa超声3周的伤口的特征在于胶原致密、血管再生的肉芽组织和完全恢复的成熟上皮。值得注意的是，在治疗2周后，暴露于0.4 MPa超声的伤口中只有一半显示出显著的肉芽组织沉积。因此，db+/db+小鼠模型可能有助于识别影响个体对脉冲超声反应的生物学变量，并加速临床转化。
Chronic wounds, including diabetic, leg, and pressure ulcers, impose a significant health care burden worldwide. Some evidence indicates that ultrasound can enhance soft tissue repair. However, therapeutic responses vary among individuals, thereby limiting clinical translation. Here, effects of pulsed ultrasound on dermal wound healing were assessed using a murine model of chronic, diabetic wounds. An ultrasound exposure system was developed to provide daily ultrasound exposures to full-thickness, excisional wounds in genetically-diabetic mice. Wounds were exposed to 1-MHz ultrasound (2 ms pulse, 100 Hz PRF, 0-0.4 MPa) for 2 or 3 weeks. Granulation tissue thickness and wound re-epithelialization increased as a function of increasing ultrasound pressure amplitude. Two weeks after injury, significant increases in granulation tissue thickness and epithelial ingrowth were observed in response to 1-MHz pulsed ultrasound at 0.4 MPa. Wounds exposed to 0.4 MPa ultrasound for 3 weeks were characterized by collagen-dense, revascularized granulation tissue with a fully restored, mature epithelium. Of note, only half of wounds exposed to 0.4 MPa ultrasound showed significant granulation tissue deposition after 2 weeks of treatment. Thus, the db+/db+ mouse model may help to identify biological variables that influence individual responses to pulsed ultrasound and accelerate clinical translation.
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影响因子：
2.9
作者：
HOGAN, RD;FRANKLIN, TD;BURKE, KM
通讯作者：
BURKE, KM
影响因子：
2.9
作者：
Bajpai A;Nadkarni S;Neidrauer M;Weingarten MS;Lewin PA;Spiller KL
通讯作者：
Spiller KL
DOI：
10.1109/t-su.1970.29552
发表时间：
1970-01-01
期刊：
IEEE TRANSACTIONS ON SONICS AND ULTRASONICS
影响因子：
--
作者：
DYSON, M;POND, JB;WARWICK, R
通讯作者：
WARWICK, R
DOI：
10.1016/0002-9378(71)90683-1
发表时间：
1971-01-01
影响因子：
9.8
作者：
LOCH, EG;FISCHER, AB;KUWERT, E
通讯作者：
KUWERT, E
影响因子：
120.7
作者：
Costa, Matthew L.;Achten, Juul;Parsons, Nick R.
通讯作者：
Parsons, Nick R.