Intranasal immunization with recombinant Toxoplasma gondii actin depolymerizing factor confers protective efficacy against toxoplasmosis in mice.
Intranasal immunization with recombinant Toxoplasma gondii actin depolymerizing factor confers protective efficacy against toxoplasmosis in mice.
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重组弓形虫肌动蛋白解聚因子鼻内免疫可赋予小鼠抗弓形虫病的保护作用
DOI：
10.1186/s12865-016-0173-9
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发表时间：
2016-10-06
期刊：
影响因子：
3
通讯作者：
Cao J
中科院分区：
文献类型：
--
作者：
Liu Z;Yin L;Li Y;Yuan F;Zhang X;Ma J;Liu H;Wang Y;Zheng K;Cao J
弓形虫是一种与艾滋病和垂直传播密切相关的机会性原虫。T.弓形虫肌动蛋白解聚因子（TgADF）在肌动蛋白细胞骨架重塑中起重要作用，是入侵宿主细胞所必需的。TgADF是一种很有前途的候选疫苗。目的观察重组TgADF蛋白（rTgADF）对T.弓形虫感染后，优化rTgADF滴鼻免疫剂量，并分析小鼠经口感染弓形虫后的存活率和组织速殖子载量。弓形虫速殖子制备、纯化rTgADF，并与小鼠抗His抗体和兔抗T.弓形虫血清经10 μg、20 μg、30 μg和40 μg rTgADF鼻内免疫后，30 μg组鼻、肠和膀胱洗液中分泌型伊加（sIgA）水平升高，血清IgG滴度升高，脾细胞增殖旺盛，IL-2和IFN-γ分泌增加。小鼠经口感染T.弓形虫存活率提高（36.36%），肝、脑速殖子载量下降（分别为67.77%和51.01%）。我们的研究结果表明，rTgADF鼻内免疫可以同时触发粘膜和全身免疫应答，并保护小鼠免受T。弓形虫感染
Toxoplasma gondii is an opportunistic protozoan closely associated with AIDS and vertical transmission. T. gondii actin depolymerizing factor (TgADF) plays an important role in actin cytoskeleton remodeling, and it is required to invade host cells. TgADF was a promising vaccine candidate. To observe the immunological changes and protective efficacy of recombinant TgADF protein (rTgADF) against T. gondii infection, we optimized the intranasal immunization dose of rTgADF and analyzed the survival rate and tachyzoite loads in mouse tissues after oral challenge with T. gondii tachyzoites. rTgADF was prepared, purified, and combined with mouse anti-His antibody and rabbit anti-T. gondii serum. After intranasal immunization with 10 μg, 20 μg, 30 μg, or 40 μg of rTgADF, the 30-μg group elicited high levels of secretory IgA (sIgA) in nasal, intestinal, and vesical washes, raised IgG titres in the sera, strong proliferation of splenocytes, and increased secretion of IL-2 and IFN-γ when compared with the control group. When the mice were orally challenged with T. gondii, an increase in the survival rate (36.36 %) and a decrease in the tachyzoite loads in the liver (67.77 %) and brain (51.01 %) were observed. Our findings demonstrate that intranasal immunization with rTgADF can simultaneously trigger mucosal and systemic immune responses and protect the mice against T. gondii infection.
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DOI：
10.1038/nrd1176
发表时间：
2003-09
期刊：
Nature reviews. Drug discovery
影响因子：
--
作者：
O'Hagan DT;Valiante NM
通讯作者：
Valiante NM
影响因子：
3.1
作者：
Sayles, PC;Gibson, GW;Johnson, LL
通讯作者：
Johnson, LL
影响因子：
2.4
作者：
BUXTON, D;INNES, EA
通讯作者：
INNES, EA
影响因子：
4.4
作者：
Kang, H;Remington, JS;Suzuki, Y
通讯作者：
Suzuki, Y
影响因子：
5.5
作者：
Eyles, JE;Spiers, ID;Alpar, HO
通讯作者：
Alpar, HO