Zonal organization of the mouse flocculus: Physiology, input, and output
Zonal organization of the mouse flocculus: Physiology, input, and output
复制标题
小鼠絮球的区域组织：生理学、输入和输出
DOI：
--
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
C. D. De Zeeuw
中科院分区：
文献类型：
--
作者：
M. Schonewille;C. Luo;T. Ruigrok;J. Voogd;M. Schmolesky;M. Rutteman;F. Hoebeek;M. de Jeu;C. D. De Zeeuw
许多物种的絮状区域已被绘制出来，但小鼠是一个明显的例外。在此，通过细胞外记录构建小鼠的功能图，然后注射生物素化葡聚糖胺示踪剂并进行热休克蛋白 25 的免疫组织化学分析。根据响应光动刺激而发生的浦肯野细胞复合体尖峰调制来识别区域。在区域 1 和区域 3 中，浦肯野细胞对围绕同侧方位角 135° 的水平轴旋转反应最好，而在区域 2 和 4 中，它们对围绕垂直轴旋转反应最好。示踪实验显示，1区浦肯野细胞投射到小脑外侧核和前庭上核的小细胞部分，3区浦肯野细胞投射到Y组和前庭上核。 2区和4区的浦肯野细胞投射到内侧前庭核的大细胞和小细胞部分，同时一些还支配外侧前庭核或舌下前核。 1 区和 3 区浦肯野细胞的攀爬纤维输入来自对侧下橄榄腹外侧生长的神经元，而 2 区和 4 区则来自对侧尾侧背帽。 1 区和 2 区（而非 3 区和 4 区）的浦肯野细胞被热休克蛋白 25 阳性标记。本研究表明，小鼠絮状细胞中的浦肯野细胞组织在具有特定功能、特定输入-输出关系和特定组织化学特征的离散区域中。 J.Comp。内罗尔。 497:670–682, 2006。© 2006 Wiley-Liss, Inc.
The zones of the flocculus have been mapped in many species with a noticeable exception, the mouse. Here, the functional map of the mouse was constructed via extracellular recordings followed by tracer injections of biotinylated‐dextran‐amine and immunohistochemistry for heat‐shock protein‐25. Zones were identified based on the Purkinje cell complex spike modulation occurring in response to optokinetic stimulation. In zones 1 and 3 Purkinje cells responded best to rotation about a horizontal axis oriented at 135° ipsilateral azimuth, whereas in zones 2 and 4 they responded best to rotation about the vertical axis. The tracing experiments showed that Purkinje cells of zone 1 projected to the parvicellular part of lateral cerebellar nucleus and superior vestibular nucleus, while Purkinje cells of zone 3 projected to group Y and the superior vestibular nucleus. Purkinje cells of zones 2 and 4 projected to the magnocellular and parvicellular parts of the medial vestibular nucleus, while some also innervated the lateral vestibular nucleus or nucleus prepositus hypoglossi. The climbing fiber inputs to Purkinje cells in zones 1 and 3 were derived from neurons in the ventrolateral outgrowth of the contralateral inferior olive, whereas those in zones 2 and 4 were derived from the contralateral caudal dorsal cap. Purkinje cells in zones 1 and 2, but not in zones 3 and 4, were positively labeled for heat‐shock protein‐25. The present study illustrates that Purkinje cells in the murine flocculus are organized in discrete zones with specific functions, specific input — output relations, and a specific histochemical signature. J. Comp. Neurol. 497:670–682, 2006. © 2006 Wiley‐Liss, Inc.
兔小脑絮球三维眼球运动的功能和解剖组织。
影响因子：
2.5
作者：
VanderSteen,J;Simpson,JI;Tan,J
通讯作者：
Tan,J