Circ-RNF121 regulates tumor progression and glucose metabolism by miR-1224-5p/FOXM1 axis in colorectal cancer.
Circ-RNF121 regulates tumor progression and glucose metabolism by miR-1224-5p/FOXM1 axis in colorectal cancer.
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DOI：
10.1186/s12935-021-02290-3
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发表时间：
2021-11-06
影响因子：
5.8
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Jiang Z;Hu H;Hu W;Hou Z;Liu W;Yu Z;Liang Z;Chen S
先前的研究报道环状RNA （circRNA）与CRC的发病机制有关。本研究旨在揭示circ-无名指蛋白121 （circ-RNF121）在结直肠癌（CRC）中的作用机制。采用实时荧光定量聚合酶链式反应（qRT-PCR）检测circ-RNF121、microRNA-1224-5p （miR-1224-5p）和叉头盒M1 （FOXM1）水平。western blot检测蛋白水平。用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）和细胞集落形成试验分析细胞增殖情况。流式细胞术检测细胞凋亡情况。用transwell法和创面愈合法观察细胞迁移和侵袭。采用葡萄糖、乳酸和ADP/ATP比值测定试剂盒检测细胞糖酵解。通过starBase在线数据库预测miR-1224-5p与circ-RNF121或FOXM1的结合关系，并通过双荧光素酶报告基因法鉴定。通过体内肿瘤形成实验揭示了circ-RNF121沉默对体内肿瘤形成的影响。与对照组相比，CRC组织或细胞中Circ-RNF121和FOXM1的表达显著上调，miR-1224-5p的表达下调。Circ-RNF121沉默抑制CRC细胞增殖、迁移、侵袭和糖酵解，但诱导细胞凋亡，miR-1224-5p抑制剂可减弱这些作用。此外，circ-RNF121作为miR-1224-5p和miR-1224-5p结合FOXM1的海绵。Circ-RNF121沉默在体内抑制肿瘤生长。此外，circ-RNF121通过被包装到外泌体中来分泌。这一发现为研究环状rna介导的结直肠癌治疗提供了新的见解。在线版本包含补充材料，可在10.1186/s12935-021-02290-3获得。
Previous studies have reported that circular RNA (circRNA) is associated with the pathogenesis of CRC. This study was designed to reveal the mechanism of circ-ring finger protein 121 (circ-RNF121) in colorectal cancer (CRC). The levels of circ-RNF121, microRNA-1224-5p (miR-1224-5p) and forkhead box M1 (FOXM1) were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Protein level was detected by western blot. Cell proliferation was analyzed by 3-(4,5-Dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and cell colony formation assays. Flow cytometry analysis was performed to investigate cell apoptosis. Cell migration and invasion were investigated by transwell and wound-healing assays. Cell glycolysis was detected using glucose, lactate and ADP/ATP ratio assay kits. The binding relationship between miR-1224-5p and circ-RNF121 or FOXM1 was predicted by starBase online database, and identified by dual-luciferase reporter assay. The impacts of circ-RNF121 silencing on tumor formation in vivo were disclosed by in vivo tumor formation assay. Circ-RNF121 and FOXM1 expression were dramatically upregulated, while miR-1224-5p expression was downregulated in CRC tissues or cells compared with control groups. Circ-RNF121 silencing repressed cell proliferation, migration, invasion and glycolysis but induced cell apoptosis in CRC, which were attenuated by miR-1224-5p inhibitor. Additionally, circ-RNF121 acted as a sponge of miR-1224-5p and miR-1224-5p bound to FOXM1. Circ-RNF121 silencing inhibited tumor growth in vivo. Furthermore, circ-RNF121 was secreted through being packaged into exosomes. The finding provided a novel insight into studying circRNA-mediated CRC therapy. The online version contains supplementary material available at 10.1186/s12935-021-02290-3.
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影响因子：
64.5
作者：
Boelens MC;Wu TJ;Nabet BY;Xu B;Qiu Y;Yoon T;Azzam DJ;Twyman-Saint Victor C;Wiemann BZ;Ishwaran H;Ter Brugge PJ;Jonkers J;Slingerland J;Minn AJ
通讯作者：
Minn AJ
DOI：
10.1146/annurev.pathol.4.110807.092222
发表时间：
2009
期刊：
Annual review of pathology
影响因子：
--
作者：
Lee YS;Dutta A
通讯作者：
Dutta A
影响因子：
7.3
作者：
Chen, Iju;Chen, Chia-Ying;Chuang, Trees-Juen
通讯作者：
Chuang, Trees-Juen
影响因子：
21.3
作者：
Laoukili, J;Kooistra, MRH;Medema, RH
通讯作者：
Medema, RH
影响因子：
28.5
作者：
Dong Y;He D;Peng Z;Peng W;Shi W;Wang J;Li B;Zhang C;Duan C
通讯作者：
Duan C