Mechanical loading affects the energy metabolism of intervertebral disc cells.
Mechanical loading affects the energy metabolism of intervertebral disc cells.
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DOI：
10.1002/jor.21430
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发表时间：
2011-11
影响因子：
2.8
通讯作者：
Huang, Chun-Yuh Charles
中科院分区：
文献类型：
--
作者：
Fernando, Hanan N.;Czamanski, Jessica;Yuan, Tai-Yi;Gu, Weiyong;Salahadin, Abdi;Huang, Chun-Yuh Charles
研究表明，机械载荷影响椎间盘（IVD）细胞的基质生物合成；然而，这种影响的途径目前尚不清楚。细胞基质生物合成是一个需要能量的过程。本研究旨在探讨静、动态压缩载荷对IVD细胞能量代谢的影响。以猪纤维环细胞（AF）和髓核细胞（NP）为实验材料，分别接种2%琼脂糖。实验组分别为15%静态压缩和0.1 Hz和1 Hz 15%应变量级动态压缩4 h。采用生化法测定培养基中ATP、乳酸、葡萄糖和一氧化氮（NO）的含量，以及细胞琼脂糖结构中ATP的含量。静态和动态加载均能提高AF细胞的总ATP含量，而仅1 Hz的动态加载能提高NP细胞的总ATP含量。房颤细胞乳酸生成和葡萄糖消耗的增加表明动态压缩促进了糖酵解产生ATP。动态加载显著增加AF和NP细胞的ATP释放和NO生成。因此，这项研究清楚地表明，静态和动态压缩载荷会影响IVD细胞的能量产生，而细胞对机械载荷的反应既依赖于细胞类型，也依赖于压缩类型。
Research has shown that mechanical loading affects matrix biosynthesis of intervertebral disc (IVD) cells; however the pathway(s) to this effect is currently unknown. Cellular matrix biosynthesis is an energy demanding process. The objective of this study was to investigate the effects of static and dynamic compressive loading on energy metabolism of IVD cells. Porcine annulus fibrosus (AF) and nucleus pulposus (NP) cells seeded in 2% agarose were used in this experiment. Experimental groups included 15% static compression and 0.1 and 1 Hz dynamic compression at 15% strain magnitude for 4 hours. ATP, lactate, glucose and nitric oxide (NO) contents in culture media, and ATP content in cell-agarose construct were measured using biochemical assays. While the total ATP content of AF cells was promoted by static and dynamic loading, only 1 Hz dynamic loading increased total ATP content of NP cells. Increases in lactate production and glucose consumption of AF cells suggest that ATP production via glycolysis is promoted by dynamic compression. ATP release and NO production of AF and NP cells were significantly increased by dynamic loading. Thus, this study clearly illustrates that static and dynamic compressive loading affect IVD cell energy production while cellular responses to mechanical loading were both cell type and compression type dependent.
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影响因子：
3.8
作者：
Gu, WY;Yao, H;Flagler, D
通讯作者：
Flagler, D
影响因子：
2.5
作者：
Choi, Kyung-Suk;Cohn, Martin J.;Harfe, Brian D.
通讯作者：
Harfe, Brian D.
影响因子：
3
作者：
Bibby, SRS;Jones, DA;Urban, JPG
通讯作者：
Urban, JPG
DOI：
10.1016/s0006-291x(05)81154-4
发表时间：
1991-10-31
影响因子：
3.1
作者：
BOGLE, RG;COADE, SB;MANN, GE
通讯作者：
MANN, GE
影响因子：
5.2
作者：
Huang, CYC;Hagar, KL;Cheung, HS
通讯作者：
Cheung, HS