Various transgenic mouse lines to study proopiomelanocortin cells in the brain stem label disparate populations of GABAergic and glutamatergic neurons.
Various transgenic mouse lines to study proopiomelanocortin cells in the brain stem label disparate populations of GABAergic and glutamatergic neurons.
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研究脑干中阿黑皮素原细胞的各种转基因小鼠品系标记了不同的 GABA 能和谷氨酸能神经元群体。
DOI：
10.1152/ajpregu.00047.2018
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Hentges,ShaneT
中科院分区：
文献类型：
--
作者：
Rau,AndrewR;Hughes,AlexanderR;Hentges,ShaneT
阿黑皮素原（POMC）激素原的产物调节镇痛、奖赏和能量平衡的各个方面;因此，下丘脑中产生POMC的神经元受到了相当大的关注。然而，在孤束核（NTS）中也有转录Pomc的细胞，尽管成年小鼠NTS中低水平的Pomc mRNA和相对缺乏POMC肽产物阻碍了对这些细胞的研究。因此，NTS POMC细胞的研究在很大程度上依赖于转基因小鼠系。在此，我们通过使用Pomc-GFP转基因小鼠来鉴定POMC细胞，以确定NTS POMC神经元的氨基酸（AA）递质表型。我们发现，表达绿色荧光蛋白（GFP）的细胞代表GABA能和谷氨酸能细胞的混合物，分别由Gad 2和囊泡Glut 2（vGlut 2）mRNA表达表示。然后，我们检查了由aPomc-Cretegene标记的POMC细胞的AA表型，并发现这些也是GABA能和谷氨酸能细胞的混合物。然而，由含有GFP和Cre的转基因标记的NTS细胞代表了三种不同Pomc-Cremouse系中不同的细胞群体。与以前的工作一致，我们无法可靠地检测PomcmRNA在NTS，尽管明确表达在下丘脑。因此，不可能确定哪种转基因工具最准确地鉴定可能表达Pomcor释放POMC肽的NTS细胞，尽管结果表明用于研究这些NTS神经元的转基因工具可以标记具有不同AA表型的不同细胞群体。
Products of the proopiomelanocortin (POMC) prohormone regulate aspects of analgesia, reward, and energy balance; thus, the neurons that produce POMC in the hypothalamus have received considerable attention. However, there are also cells in the nucleus of the solitary tract (NTS) that transcribePomc, although low levels ofPomcmRNA and relative lack of POMC peptide products in the adult mouse NTS have hindered the study of these cells. Therefore, studies of NTS POMC cells have largely relied on transgenic mouse lines. Here, we set out to determine the amino acid (AA) transmitter phenotype of NTS POMC neurons by usingPomc-Gfptransgenic mice to identify POMC cells. We found that cells expressing the green fluorescent protein (GFP) represent a mix of GABAergic and glutamatergic cells as indicated byGad2and vesicularGlut2(vGlut2) mRNA expression, respectively. We then examined the AA phenotype of POMC cells labeled by aPomc-Cretransgene and found that these are also a mix of GABAergic and glutamatergic cells. However, the NTS cells labeled by theGfp- andCre-containing transgenes represented distinct populations of cells in three differentPomc-Cremouse lines. Consistent with previous work, we were unable to reliably detectPomcmRNA in the NTS despite clear expression in the hypothalamus. Thus, it was not possible to determine which transgenic tool most accurately identifies NTS cells that may expressPomcor release POMC peptides, although the results indicate the transgenic tools for study of these NTS neurons can label disparate populations of cells with varied AA phenotypes.
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影响因子：
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作者：
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