A multidimensional micro gas chromatograph employing a parallel separation multi-column chip and stop-flow μGC × μGCs configuration.
A multidimensional micro gas chromatograph employing a parallel separation multi-column chip and stop-flow μGC × μGCs configuration.
复制标题
采用平行分离多柱芯片和停流μGC×μGCs配置的多维微型气相色谱仪。
作者：
B. Chen;T. Hung;R. Jian;Chia
设计、制作了一种双芯片多维微型气相色谱模块，并对其性能进行了评价。采用深反应离子刻蚀（DRIE）工艺，在宽100 μm × 250 μm的矩形通道硅片上制作了柱状芯片。用于第一GC维度的柱芯片为3 m长，并且涂覆有聚二甲基硅氧烷（DB-1）作为固定相。第二维芯片上的柱以与第一芯片相同的宽度和深度蚀刻，但是流动通道在相同尺寸的硅芯片上分成三个1 m长的平行柱（即，3 cm × 3 cm）。第二个芯片上的这三个平行柱相应地用聚环氧乙烷（DB-Wax）、三氟丙基聚甲基硅氧烷（OV-210）和氰丙基甲基苯基甲基聚硅氧烷（OV-225）涂覆，以提供多样化的色谱保留。这两个芯片通过停流配置连接，以同时生成每次分析的多个二维气相色谱图。这种停流μGC × μ GC设计允许第一个柱作为预分离器和第二个平行分离芯片的测序进样器。使用该μGC × μ GC模块测试了15种沸点范围为80-131 °C的具有不同官能团的挥发性有机化合物。同时生成了三个离散的二维色谱图，证明了在微芯片色谱中同时将GC × GC与平行分离GC相结合的优势。每个洗脱峰在柱中的总行进长度仅为4 m，并且通过三重二维色谱图之间的交叉参考实现完全分辨的分离。
A dual-chip, multidimensional micro gas chromatographic module was designed, built and evaluated. Column chips were fabricated on a silicon wafer with an etched rectangular channel 100 μm (width) × 250 μm (depth) using a deep reactive ion etching (DRIE) process. The column chip for the first GC dimension was 3 m long and was coated with polydimethylsiloxane (DB-1) as the stationary phase. The columns on the second dimensional chip were etched with the same width and depth as the first chip, but the flow channel was split into three parallel columns, 1 m long, on the same sized silicon chip (i.e., 3 cm × 3 cm). These three parallel columns on the second chip were coated with polyethylene oxide (DB-Wax), trifluoropropylpolymethylsilicone (OV-210) and cyanopropylmethylphenylmethylpolysilicone (OV-225), accordingly, in order to provide diversified chromatographic retention. These two chips were connected via a stop-flow configuration to simultaneously generate multiple two-dimensional gas chromatograms for every analysis. This stop-flow μGC × μGCs design allowed the first column to function as a pre-separator and as a sequencing injector for the second parallel-separation chip. Fifteen volatile organic compounds with boiling points that ranged from 80-131 °C with various functional groups were tested using this μGC × μGCs module. Three discrete 2-D chromatograms were generated simultaneously, which demonstrated the advantages of simultaneously combining GC × GC with parallel separation GCs in microchip chromatography. The total traveling length in the column was only 4 m for each eluted peak and fully resolved separation was achieved through the cross reference among triplet 2-D chromatograms.
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影响因子：
7.4
作者：
Lu, CJ;Whiting, J;Zellers, ET
通讯作者：
Zellers, ET
影响因子：
6.1
作者：
Lu, CJ;Steinecker, WH;Zellers, ET
通讯作者：
Zellers, ET
影响因子：
7.4
作者：
J. Whiting;C. Lu;E. Zellers;R. Sacks
通讯作者：
J. Whiting;C. Lu;E. Zellers;R. Sacks
影响因子：
7.4
作者：
W. H. Steinecker;M. Rowe;E. Zellers
通讯作者：
W. H. Steinecker;M. Rowe;E. Zellers
使用第二根色谱柱的脉冲加热，通过串联色谱柱实现 GC 的可编程选择性。
影响因子：
7.4
作者：
Whiting,Joshua;Sacks,Richard
通讯作者：
Sacks,Richard