Novel Strategy to Fabricate PLA/Au Nanocomposites as an Efficient Drug Carrier for Human Leukemia Cells in Vitro.
Novel Strategy to Fabricate PLA/Au Nanocomposites as an Efficient Drug Carrier for Human Leukemia Cells in Vitro.
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DOI：
10.1007/s11671-010-9762-3
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发表时间：
2011-12
影响因子：
--
通讯作者：
Chen, Baoan
中科院分区：
文献类型：
--
作者：
Li, Jingyuan;Chen, Chen;Wang, Xuemei;Gu, Zhongze;Chen, Baoan
聚乳酸聚合物具有良好的生物降解性和消除安全性，在生物医学领域具有广阔的应用前景。在这项研究中，我们探索了由聚乳酸纳米纤维和金纳米颗粒组成的新型纳米复合材料作为潜在的药物载体在靶向癌细胞中的有效药物输送的生物应用。结果表明，抗癌药物柔红霉素可以有效地自组装到聚乳酸/金纳米复合材料表面，并且通过四甲基偶氮唑盐比色法和共聚焦荧光显微镜研究可以明显地观察到与柔红霉素偶联的聚乳酸/金纳米复合材料对药物敏感的K562和耐药白血病K562/AO2细胞的协同增强作用。这些观察表明，新的纳米复合材料可以很容易地诱导柔红霉素在靶细胞中蓄积和摄取，并增加药物的细胞毒性。特别是聚乳酸/金纳米复合材料能显著促进柔红霉素在耐药K562/AO2细胞中的药物吸收，并有效抑制癌细胞的增殖。这增加了利用聚乳酸/金纳米复合材料作为一种新的有效的添加剂来抑制耐药的可能性，从而作为一种新的策略来灵敏地示踪相应的癌细胞。
Poly (lactic acid) (PLA) polymer has the promising applications in the biomedical field because of its biodegradability and safe elimination. In this study, we have explored the bio-application of new nanocomposites composed with PLA nanofibers and Au nanoparticles as the potential drug carrier for an efficient drug delivery in target cancer cells. The results demonstrated that the anticancer drug daunorubicin could be efficiently self-assembled on the surface of PLA/Au nanocomposites and the synergistic enhancement of PLA/Au nanocomposites conjugated with daunorubicin into drug-sensitive K562 and drug-resistant leukemia K562/AO2 cells could be obviously observed by MTT assay and confocal fluorescence microscopy studies. These observations suggest that the new nanocomposites could readily induce daunorubicin to accumulate and uptake in target leukemia cells and increase the drug's cytotoxicity. Especially, the PLA/Au nanocomposites could significantly facilitate the cellular drug absorbtion of daunorubicin into drug-resistant K562/AO2 cells and efficiently inhibit the cancer cell proliferation. This raised the possibility to utilize the PLA/Au nanocomposites as a new effective additive agent to inhibit the drug resistance and thus as a novel strategy to sensitively track the respective cancer cells.
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