Exosomes Derived from Dendritic Cells Treated with Schistosoma japonicum Soluble Egg Antigen Attenuate DSS-Induced Colitis.
Exosomes Derived from Dendritic Cells Treated with Schistosoma japonicum Soluble Egg Antigen Attenuate DSS-Induced Colitis.
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用日本血吸虫可溶性卵抗原处理的树突状细胞衍生的外泌体可减轻 DSS 诱导的结肠炎
DOI：
10.3389/fphar.2017.00651
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wu Z
中科院分区：
文献类型：
--
作者：
Wang L;Yu Z;Wan S;Wu F;Chen W;Zhang B;Lin D;Liu J;Xie H;Sun X;Wu Z
外泌体是30 - 150纳米的小膜泡，通过细胞释放到细胞外介质中，作为一种细胞间通讯方式发挥作用。树突状细胞（DC）衍生的外泌体可调节免疫反应并预防自身免疫性疾病的发生。此外，日本血吸虫卵在结肠炎小鼠模型中显示出调节作用。因此，我们假设用日本血吸虫可溶性虫卵抗原（SEA；SEA处理的DC外泌体）处理的DC衍生的外泌体可用于治疗炎症性肠病（IBD）。从用SEA处理或未处理的DC上清液中纯化外泌体，并通过透射电子显微镜、蛋白质印迹和纳米颗粒跟踪分析进行鉴定。通过在饮用水中给予葡聚糖硫酸钠（DSS，5.0%，重量/体积）诱导急性结肠炎。从第0天到第6天通过腹腔注射（i.p.；每只小鼠50μg）进行外泌体治疗。根据体重减轻、粪便类型和出血情况计算临床评分。测量结肠长度作为炎症的间接标志物，并确定结肠宏观特征。用SEA处理的DC外泌体治疗后，DSS诱导的结肠炎小鼠的体重减轻和疾病活动指数显著降低。此外，SEA处理的DC外泌体治疗的结肠炎小鼠的结肠长度得到改善，其平均结肠宏观评分降低。此外，组织学检查和组织学评分表明，SEA处理的DC外泌体可防止急性DSS诱导的结肠炎小鼠的结肠损伤。这些结果表明，SEA处理的DC外泌体比DC外泌体更有效地减轻急性DSS诱导的结肠炎小鼠的严重程度。目前的研究表明，SEA处理的DC外泌体可能作为一种治疗IBD的新方法是有用的。
Exosomes are 30–150 nm small membrane vesicles that are released into the extracellular medium via cells that function as a mode of intercellular communication. Dendritic cell (DC)-derived exosomes modulate immune responses and prevent the development of autoimmune diseases. Moreover, Schistosoma japonicum eggs show modulatory effects in a mouse model of colitis. Therefore, we hypothesized that exosomes derived from DCs treated with S. japonicum soluble eggs antigen (SEA; SEA-treated DC exosomes) would be useful for treating inflammatory bowel disease (IBD). Exosomes were purified from the supernatant of DCs treated or untreated with SEA and identified via transmission electron microscopy, western blotting and NanoSight. Acute colitis was induced via the administration of dextran sulfate sodium (DSS) in drinking water (5.0%, wt/vol). Treatment with exosomes was conducted via intraperitoneal injection (i.p.; 50 μg per mouse) from day 0 to day 6. Clinical scores were calculated based on weight loss, stool type, and bleeding. Colon length was measured as an indirect marker of inflammation, and colon macroscopic characteristics were determined. Body weight loss and the disease activity index of DSS-induced colitis mice decreased significantly following treatment with SEA-treated DC exosomes. Moreover, the colon lengths of SEA-treated DC exosomes treated colitis mice improved, and their mean colon macroscopic scores decreased. In addition, histologic examinations and histological scores showed that SEA-treated DC exosomes prevented colon damage in acute DSS-induced colitis mice. These results indicate that SEA-treated DC exosomes attenuate the severity of acute DSS-induced colitis mice more effectively than DC exosomes. The current work suggests that SEA-treated DC exosomes may be useful as a new approach to treat IBD.
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联邦交易体：真正提供的工程治疗生物纳米颗粒。
DOI：
10.3390/ph6050659
发表时间：
2013
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
Marcus ME;Leonard JN
通讯作者：
Leonard JN
源自人脐带间充质干细胞的外泌体可缓解小鼠炎症性肠病
影响因子：
--
作者：
Mao F;Wu Y;Tang X;Kang J;Zhang B;Yan Y;Qian H;Zhang X;Xu W
通讯作者：
Xu W
影响因子：
7.2
作者：
Menghini, Luigi;Ferrante, Claudio;Brunetti, Luigi
通讯作者：
Brunetti, Luigi
影响因子：
4.9
作者：
Hanauer, SB
通讯作者：
Hanauer, SB
影响因子：
2.2
作者：
Lutz, MB;Kukutsch, N;Schuler, G
通讯作者：
Schuler, G