Prevalence of alternative splicing choices in Arabidopsis thaliana.
Prevalence of alternative splicing choices in Arabidopsis thaliana.
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DOI：
10.1186/1471-2229-10-102
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发表时间：
2010-06-04
影响因子：
5.3
通讯作者：
Loraine AE
中科院分区：
文献类型：
--
作者：
English AC;Patel KS;Loraine AE
Around 14% of protein-coding genes of Arabidopsis thaliana genes from the TAIR9 genome release are annotated as producing multiple transcript variants through alternative splicing. However, for most alternatively spliced genes in Arabidopsis, the relative expression level of individual splicing variants is unknown. We investigated prevalence of alternative splicing (AS) events in Arabidopsis thaliana using ESTs. We found that for most AS events with ample EST coverage, the majority of overlapping ESTs strongly supported one major splicing choice, with less than 10% of ESTs supporting the minor form. Analysis of ESTs also revealed a small but noteworthy subset of genes for which alternative choices appeared with about equal prevalence, suggesting that for these genes the variant splicing forms co-occur in the same cell types. Of the AS events in which both forms were about equally prevalent, more than 80% affected untranslated regions or involved small changes to the encoded protein sequence. Currently available evidence from ESTs indicates that alternative splicing in Arabidopsis occurs and affects many genes, but for most genes with documented alternative splicing, one AS choice predominates. To aid investigation of the role AS may play in modulating function of Arabidopsis genes, we provide an on-line resource (ArabiTag) that supports searching AS events by gene, by EST library keyword search, and by relative prevalence of minor and major forms.
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影响因子：
4.4
作者：
Pao, Sheng-Ying;Lin, Win-Li;Hwang, Ming-Jing
通讯作者：
Hwang, Ming-Jing
DOI：
10.1093/bioinformatics/btp472
发表时间：
2009-10-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Nicol JW;Helt GA;Blanchard SG Jr;Raja A;Loraine AE
通讯作者：
Loraine AE
影响因子：
7.2
作者：
Hori, K;Watanabe, Y
通讯作者：
Watanabe, Y
影响因子：
4.4
作者：
Schindler, Stefanie;Szafranski, Karol;Hiller, Michael;Ali, Gul Shad;Palusa, Saiprasad G.;Backofen, Rolf;Platzer, Matthias;Reddy, Anireddy S. N.
通讯作者：
Reddy, Anireddy S. N.
影响因子：
3.7
作者：
Carey, Charles C.;Gorman, Kristen F.;Rutherford, Suzannah
通讯作者：
Rutherford, Suzannah