Paper Biosensor for the Detection of NT-proBNP Using Silver Nanodisks as Electrochemical Labels.
Paper Biosensor for the Detection of NT-proBNP Using Silver Nanodisks as Electrochemical Labels.
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DOI：
10.3390/nano12132254
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发表时间：
2022-06-30
期刊：
影响因子：
5.3
通讯作者：
Crooks, Richard M.
中科院分区：
文献类型：
--
作者：
Peng, Yi;Raj, Nikhil;Strasser, Juliette W.;Crooks, Richard M.
我们报道了使用直径为50±8 nm，厚度为8±2 nm的银纳米片（AgNDs）作为电化学标记，用于检测心力衰竭生物标志物NT-proBNP的模型金属免疫分析。该检测方法是基于电化学激活的电交换（GE），然后使用阳极溶出伏安法（ASV）检测银。在模型和NT-proBNP金属免疫分析的动态范围方面，AgNDs标记优于Ag纳米立方和Ag纳米球。模型复合材料的线性动态范围为1.5至30.0 pM agnd。当AgND标签用于NT-proBNP分析时，动态范围为0.03-4.0 nM NT-proBNP。后一个范围完全重叠心力衰竭的风险分层范围从下午53点到下午590点。AgNDs的性能提高是纳米磁盘特定GE机制的结果。具体来说，GE在agnd的表面是完整的，留下了一个由Ag和Au组成的不完全交换的环结构。
We report on the use of silver nanodisks (AgNDs), having a diameter of 50 ± 8 nm and a thickness of 8 ± 2 nm, as electrochemical labels for the detection of a model metalloimmunoassay for the heart failure biomarker NT-proBNP. The detection method is based on an electrochemically activated galvanic exchange (GE) followed by the detection of Ag using anodic stripping voltammetry (ASV). The AgNDs labels are superior to Ag nanocubes and Ag nanospheres in terms of the dynamic range for both the model and NT-proBNP metalloimmunoassays. The linear dynamic range for the model composite is 1.5 to 30.0 pM AgNDs. When AgND labels are used for the NT-proBNP assay, the dynamic range is 0.03–4.0 nM NT-proBNP. The latter range fully overlaps the risk stratification range for heart failure from 53 pM to 590 pM. The performance improvement of the AgNDs is a result of the specific GE mechanism for nanodisks. Specifically, GE is complete across the face of the AgNDs, leaving behind an incompletely exchanged ring structure composed of both Ag and Au.
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影响因子：
8.9
作者：
Peng Y;Rabin C;Walgama CT;Pollok NE;Smith L;Richards I;Crooks RM
通讯作者：
Crooks RM
影响因子：
8.9
作者：
Pollok NE;Peng Y;Rabin C;Richards I;Crooks RM
通讯作者：
Crooks RM
影响因子：
1.6
作者：
Karabulut, A;Kaplan, A;Toprak, N
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Toprak, N
影响因子：
8.9
作者：
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通讯作者：
Crooks, Richard M.
影响因子：
6.4
作者：
Ghosh, Tanmay;Satpati, Biswarup;Senapati, Dulal
通讯作者：
Senapati, Dulal